FGF2 Protéine

N° du produit ABIN2129215

Détail du produit

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Séquence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Attributs du produit: Purified recombinant Human FGF basic protein
Expression System: E.coli
Bioactivity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ? 0.5 ng/mL, corresponding to a specific activity of ? 2 x 10^6 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ? 0.1 ng/mL, corresponding to a specific activity of ? 1 x 10^7 units/mg.

Pureté: > 95 % pure

niveau d'endotoxine: < 0.1 ng per μg (1 EU/μg).

Information d'application

Indications d'application: Each Investigator should determine their own optimal working dilution for specific applications.

Commentaires:

Biological activity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is approx. 0.5 ng/mL, corresponding to a specific activity of approx. 2 x 10^6 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is approx. 0.1 ng/mL, corresponding to a specific activity of approx. 1 x 10^7 units/mg.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1 - 1.0 mg/mL.

Buffer: Lyophilized from 5 mM Tris, pH 7.6 with 155 mM NaCl.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Détail du antigène

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Autre désignation: FGF Basic (FGF2 Produits)

Synonymes: BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein

Sujet: FGF basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Alternative Names: FGF-2 protein, Prostatropin protein, Fibroblast Growth Factor-basic protein, HBGF-2 protein

Poids moléculaire: 17.2 kDa

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins