FGF2 Protéine

N° du produit ABIN2129216

Détail du produit

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Séquence: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

Attributs du produit: Purified recombinant Human FGF basic protein
Expression System: E.coli
Bioactivity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is

Pureté: > 98 % pure

Information d'application

Indications d'application: Each Investigator should determine their own optimal working dilution for specific applications.

Commentaires:

Biological activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/mL, corresponding to a specific activity of 2,000,000 Units/mg.

Procédure de l'essai:

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in distilled water to at least 100 μg/mL.

Buffer: Supplied in lyophilized form containing 10 mM Acetic acid,

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Référence

Czekanska, Ralphs, Alini, Stoddart: "Enhancing inflammatory and chemotactic signals to regulate bone regeneration." dans: European cells & materials, Vol. 28, pp. 320-34, (2014) (PubMed).

Détail du antigène

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Autre désignation: FGF Basic (FGF2 Produits)

Synonymes: BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein

Sujet: FGF basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Alternative Names: FGF-2 protein, HBGH-2 protein, HBGF-2 protein, FGF-b protein, Fibroblast Growth Factor Basic protein, Prostatropin protein

Poids moléculaire: 17,353 Da

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins