This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
EPCAM
Origine: Rat
Hôte: HEK-293 Cells
Recombinant
> 97 % as determined by SDS-PAGE
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Robertson, Lopez-Anton, Gurjar, Khalique, Khalaf, Clerkin, Leydon, Parker-Manuel, Raeside, Payne, Jones, Seymour, Cawood: "Development of a novel mammalian display system for selection of antibodies against membrane proteins." dans: The Journal of biological chemistry, Vol. 295, Issue 52, pp. 18436-18448, (2020) (PubMed).
Abali, Broekmaat, Tibbe, Schasfoort, Zeune, Terstappen: "A microwell array platform to print and measure biomolecules produced by single cells." dans: Lab on a chip, Vol. 19, Issue 10, pp. 1850-1859, (2019) (PubMed).
Stojanović, Baumgartner, van der Velden, Terstappen, Schasfoort: "Modeling single cell antibody excretion on a biosensor." dans: Analytical biochemistry, Vol. 504, pp. 1-3, (2017) (PubMed).
Xu, Lee, Tran, Heibeck, Wang, Yang, Stafford, Steiner, Sato, Hallam, Yin: "Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system." dans: mAbs, Vol. 7, Issue 1, pp. 231-42, (2015) (PubMed).
Zielonka, Empting, Könning, Grzeschik, Krah, Becker, Dickgießer, Kolmar: "The Shark Strikes Twice: Hypervariable Loop 2 of Shark IgNAR Antibody Variable Domains and Its Potential to Function as an Autonomous Paratope." dans: Marine biotechnology (New York, N.Y.), Vol. 17, Issue 4, pp. 386-92, (2015) (PubMed).
Zielonka, Weber, Becker, Doerner, Christmann, Christmann, Uth, Fritz, Schäfer, Steinmann, Empting, Ockelmann, Lierz, Kolmar: "Shark Attack: high affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation." dans: Journal of biotechnology, Vol. 191, pp. 236-45, (2014) (PubMed).
EpCAM is also known as CO171A, EGP, EGP40,GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.