IL-3 Protein (AA 27-169)

N° du produit ABIN2666554

Cette protéine Recombinant IL-3 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour IL-3 Protein (AA 27-169) (ABIN2666554)

Antigène: IL-3 (Interleukin 3 (IL-3))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Pureté: > 95 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 27-169

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 = 1 - 5 ng/ml, corresponding to a specific activity of 0.2 â€" 1.0 x 106 units/mg, as determined by the dose dependent stimulation of a M-NFS-60 cell proliferation assay.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS pH 7.2.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: IL-3

Sujet: Interleukin 3, also known as IL-3, was initially described in the supernatant of cultures of viral-infected murine spleen cells. Mature rat IL-3 surprisingly shows low homology (54 % ) with its murine counterpart. IL-3 is the most potent growth factor for basophils followed by the granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and release of the cells. IL-3 is highly expressed by mast cells. Rapid autocrine production of large amounts of the cytokine is responsible for mast cell IgE-induced survival in the absence of antigen. In addition, IL-3 and its receptors are expressed in neuronal progenitors and neurons. IL-3 has been associated to human brain volume variation by regulating proliferation and survival of neural progenitors. It has also been implicated in the pathogenesis of several chronic inflammatory diseases, which include asthma, atherosclerosis, and neurodegenerative disorders such as multiple sclerosis.

Poids moléculaire: The 144 amino acid recombinant protein has a predicted molecular mass of approximately 16.3 kDa. The protein migrates at approximately 18 kDa in DTT-reducing conditions and 16 kDa in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy