Osteoprotegerin Protein (AA 22-401, C-Term)

N° du produit ABIN2666865

Détail du produit

Antigène: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 22-401, C-Term

Origine: Souris

Source: HEK-293 Cells

Application: Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS)

Pureté: > 95 % , as determined by Coomassie stained SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 = 30 - 90 ng/ml, corresponding to a specific activity of 1 - 3 x 104 units/mg, as determined by inhibition of cytotoxic effect of human TRAIL on L929 cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Autre désignation: TNFRSF11B (TNFRSF11B Produits)

Synonymes: CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, OCIF Protein, OPG Protein, TR1 Protein, Opg Protein, ocif Protein, opg Protein, tr1 Protein, TNF superfamily member 11 Protein, TNF receptor superfamily member 11b Protein, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) Protein, TNF receptor superfamily member 11B Protein, tumor necrosis factor receptor superfamily member 11b S homeolog Protein, tumor necrosis factor receptor superfamily, member 11b Protein, TNFSF11 Protein, TNFRSF11B Protein, Tnfrsf11b Protein, tnfrsf11b.S Protein

Classe de substances: Chemical

Sujet: TNFRSF11B is a member of the TNFR family, which lacks the transmembrane domain and acts as a secreted decoy receptor with no direct signaling capacity. It plays a key role in the physiological regulation of osteoclastic bone resorption, postnatal bone formation, and pathological calcification of arteries. TNFRSF11B binds to its natural ligand, RANKL (OPGL), which prevents the interaction of RANKL with its receptor, present on osteoclasts, therefore inhibiting osteoclast differentiation, activation, and survival. Overexpression of TNFRSF11B in transgenic mice leads to deep osteoporosis secondary to an almost total lack of osteoclasts. In addition, TNFRSF11B-deficient mice develop early onset osteoporosis and calcification of the aorta and renal arteries, sites which have endogenous TNFRSF11B expression in normal animals. EBF family member, EBF2 activates TNFRSF11B gene transcription in a synergistic manner with Wnt/β-catenin signaling transcription factor LEF1. In addition to RANKL, TNFRSF11B binds and neutralizes the pro-apoptotic actions of TNF-related apoptosis-inducing ligand (TRAIL) expressed by vascular smooth muscle cells (VSMCs) and T cells. Lastly, TNFRSF11B is involved in B cell development and function. High levels of TNFRSF11B have been detected in serum of patients with cardiovascular diseases, diabetes, and chronic renal failure.

Poids moléculaire: The 620 amino acid recombinant protein has a predicted molecular mass of approximately 70.6 kDa. The DTT-reduced and non-reduced protein migrate at approximately 100 kDa and 200 kDa, respectively, by SDS-PAGE. The predicted N-terminal amino acid is Glu.