VCAM1 Protein (AA 25-698, C-Term) (Fc Tag)

N° du produit ABIN2666869

Détail du produit

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 25-698, C-Term

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette VCAM1 protéine est marqué à la Fc Tag.

Application: ELISA, Flow Cytometry (FACS)

Pureté: > 95 % , as determined by Coomassie stained SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: Immobilized human VCAM-1-Fc chimera is able to bind U937 cells (5x104 /well, 1 h at room temperature) in a dose dependent manner. Over 80% of the cells were able to bind human VCAM-1 at a dose greater than 5.0 μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Concentration: 100 μg/mL

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Autre désignation: VCAM-1 (VCAM1 Produits)

Synonymes: CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein

Sujet: VCAM-1, vascular cell adhesion molecule 1, is a member of the immunoglobulin superfamily. It is expressed on both large and small blood vessels only after the endothelial cells are stimulated by cytokines, such as IL-4, TNF-α, and IL-1. Within the Ig superfamily, VCAM-1 belongs to a subfamily of cell surface molecules including ICAM-1, -2, and -3, and MAdCAM-1, that bind leukocyte integrin ligands. Members of this family are distinguished by the presence of four cysteine residues instead of the usual two in domains important for counter-receptor binding. Structurally, VCAM-1 exists in two alternatively spliced forms on endothelial cells, a major form comprised of seven Ig-like domains, and a minor form with six Ig-like domains. It has been further demonstrated that domains 1 and 4 of VCAM-1 are critical for binding to its counter-receptor VLA-4 (very late antigen 4, α4β1), while domains 1-3 are important for cell adhesion and costimulation of T cells. The interaction of VCAM-1 and VLA-4 appears to play a role in a number of developmental pathways and inflammatory functions. In addition, it was shown that by interacting with VLA-4, VCAM-1 recruits monocytic osteoclast progenitors and elevates local osteoclast activity. Antibodies against VCAM-1 and integrin α4 effectively inhibit bone metastasis progression and preserve bone structure. These findings establish VCAM-1 as a promising target for the prevention and inhibition of metastatic recurrence in bone.

Poids moléculaire: The 914 amino acid recombinant protein with a C-terminal human IgG1 Fc tag has a predicted molecular mass of approximately 101.4 kDa. The protein migrates at approximately 130 kDa by SDS-PAGE in DTT-reducing conditions and approximately 260 kDa in non-red

Pathways: Carbohydrate Homeostasis