SMARCA4 Protein (AA 1448-1569) (GST tag)

N° du produit ABIN2669726

Détail du produit

Antigène: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Type de proteíne: Recombinant

Attributs du protein: AA 1448-1569

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SMARCA4 protéine est marqué à la GST tag.

Application: Binding Studies (Bind), Screening Assay (ScA)

Attributs du produit: The peptide corresponding to amino acids 1448-1569 that contains the bromodomain sequences of SMARCA4 (accession number NP_003063.2) was expressed in E. coli and contains an N-terminal GST tag with an observed molecular weight of 41.6 kDa. It shows binding specificity for acetylated H2BK5, H3K14 and H3K9/14.

Information d'application

Indications d'application: Recombinant SMARCA4 / BRG1 (1448-1569), GST-tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Autre désignation: SMARCA4 / BRG1 (SMARCA4 Produits)

Synonymes: SMARCA4 Protein, BAF190 Protein, BAF190A Protein, BRG1 Protein, MRD16 Protein, RTPS2 Protein, SNF2 Protein, SNF2L4 Protein, SNF2LB Protein, SWI2 Protein, hSNF2b Protein, brg-1 Protein, Brg1 Protein, HP1-BP72 Protein, SNF2beta Protein, SW1/SNF Protein, b2b692Clo Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 Protein, SMARCA4 Protein, Smarca4 Protein

Sujet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA4 contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA4 is part of the large ATP-dependent chromatin remodeling complex SNF/SWI which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Gene mutation causes Rhabdoid Tumor Predisposition Syndrome Type 2. SMARCA4 functions as a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It also interacts with glucocorticoid receptor (GR), TOPBP1 and progesterone receptor (PR) and is a component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Somatic mutations of SMARCA4 have been detected in some cancer cell lines and loss of SMARCA4 is associated with decreased survival in cancer patients.

Poids moléculaire: 41.6 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance