HECW2 Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2727068

Détail du produit

Antigène: HECW2 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette HECW2 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human NEDL2 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: HECW2 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2))

Autre désignation: Nedl2 (HECW2 Produits)

Synonymes: HECW2 Protein, NEDL2 Protein, A730039N16Rik Protein, D030049F17Rik Protein, Gm971 Protein, Nedl2 Protein, mKIAA1301 Protein, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 Protein, E3 ubiquitin-protein ligase HECW2 Protein, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b Protein, Hecw2 Protein, HECW2 Protein, LOC531691 Protein, hecw2b Protein, hecw2 Protein

Sujet: This gene encodes a member of a family of E3 ubiquitin ligases which plays an important role in the proliferation, migration and differentiation of neural crest cells as a regulator of glial cell line-derived neurotrophic factor (GDNF)/Ret signaling. This gene also plays an important role in angiogenesis through stabilization of endothelial cell-to-cell junctions as a regulator of angiomotin-like 1 stability. The encoded protein contains an N-terminal calcium/lipid-binding (C2) domain involved in membrane targeting, two-four WW domains responsible for cellular localization and substrate recognition, and a C-terminal homologous with E6-associated protein C-terminus (HECT) catalytic domain. Naturally occurring mutations in this gene are associated with neurodevelopmental delay, hypotonia, and epilepsy. The decreased expression of this gene in the aganglionic colon is associated with Hirschsprung&aposs disease. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 175.6 kDa

NCBI Accession: NP_065811