OCT4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

N° du produit ABIN2727872

Détail du produit

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Type de proteíne: Recombinant

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette OCT4 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human OCT3/4 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Soufi, Garcia, Jaroszewicz, Osman, Pellegrini, Zaret: "Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming." dans: Cell, Vol. 161, Issue 3, pp. 555-68, (2015) (PubMed).

Détail du antigène

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Autre désignation: Oct3/4 (POU5F1 Produits)

Synonymes: OCT3 Protein, OCT4 Protein, OTF-3 Protein, OTF3 Protein, OTF4 Protein, Oct-3 Protein, Oct-4 Protein, POU5F1 Protein, gp9 Protein, gp-9 Protein, oct4 Protein, pou2 Protein, POU-2 Protein, cb197 Protein, spg/pou2 Protein, chunp6868 Protein, wu:fd18d06 Protein, NF-A3 Protein, Oct-3/4 Protein, Oct3 Protein, Oct3/4 Protein, Oct4 Protein, Otf-3 Protein, Otf-4 Protein, Otf3 Protein, Otf3-rs7 Protein, Otf3g Protein, Otf4 Protein, POU class 5 homeobox 1 Protein, POU domain, class 5, transcription factor 3 Protein, POU domain, class 5, transcription factor 1 Protein, POU5F1 Protein, pou5f3 Protein, Pou5f1 Protein

Sujet: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing&aposs sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Poids moléculaire: 38.4 kDa

NCBI Accession: NP_002692

Pathways: Stem Cell Maintenance