PAM Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

N° du produit ABIN2728195

Détail du produit

Antigène: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Transcript Variant 3

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette PAM protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human PAM (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: The specific activity of PAM was determined by measuring the product D-Tyr-Val-NH2 formation from a conversion of D-Tyr-Val-Gly. The reaction was carried out at 37C for 60min in the buffer containing 50 mM MES, pH6.0, 1 μM CuCl2, 2000 units/ml of Catalase, 5 mM L-ascorbic acid, and 50 uM of D-Tyr-Val-Gly as the substrate

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Autre désignation: Pam (PAM Produits)

Synonymes: PAL Protein, PHM Protein, pal Protein, phm Protein, pamb Protein, CG3832 Protein, Dmel\\CG3832 Protein, dPHM Protein, AE-I Protein, PAL-A Protein, PHM-A Protein, pama Protein, si:dkeyp-35f12.2 Protein, peptidylglycine alpha-amidating monooxygenase Protein, peptidylglycine alpha-amidating monooxygenase S homeolog Protein, Peptidylglycine-alpha-hydroxylating monooxygenase Protein, peptidylglycine alpha-amidating monooxygenase L homeolog Protein, PAM Protein, Pam Protein, pam.S Protein, CHLREDRAFT_150435 Protein, sce7070 Protein, pam Protein, Phm Protein, pam.L Protein

Sujet: Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. [UniProtKB/Swiss-Prot Function]

Poids moléculaire: 94.2 kDa

NCBI Accession: NP_620176