PPP1R12A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

N° du produit ABIN2729469

Détail du produit

Antigène: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Type de proteíne: Recombinant

Attributs du protein: Transcript Variant 2

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette PPP1R12A protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human PPP1R12A / MYPT1 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Autre désignation: Ppp1r12a,mypt1 (PPP1R12A Produits)

Synonymes: 1200015F06Rik Protein, 5730577I22Rik Protein, AA792106 Protein, AV099298 Protein, D10Ertd625e Protein, Mypt1 Protein, M130 Protein, MBS Protein, MYPT1 Protein, M110 Protein, MBSP Protein, protein phosphatase 1, regulatory (inhibitor) subunit 12A Protein, protein phosphatase 1 regulatory subunit 12A Protein, protein phosphatase 1, regulatory subunit 12A Protein, Ppp1r12a Protein, PPP1R12A Protein

Sujet: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 115.1 kDa

NCBI Accession: NP_001137357

Pathways: M Phase