VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))
Type de proteíne
Recombinant
Activité biologique
Active
Attributs du protein
Transcript Variant 2
Origine
Souris
Source
Escherichia coli (E. coli)
Application
Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human VEGF-A (transcript variant 2) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 98 % , as determined by Coomassie stained SDS-PAGE.
niveau d'endotoxine
Less than 0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 10^6 units/mg, determined by the dose dependent stimulation of HUVEC cells proliferation.
VEGFA
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
VEGFA
Origine: Souris
Hôte: Yeast (Pichia pastoris)
Recombinant
> 95 % as determined by SDS-PAGE
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Concentration
> 50 μg/mL
Buffer
5 mM Citric Acid, 5 mM NaH2PO4, 150 mM NaCl, pH 4.0
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Antigène
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))