NADK Protein (His tag)
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- Antigène Voir toutes NADK Protéines
- NADK (NAD Kinase (NADK))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Mycobacterium tuberculosis
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette NADK protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Attributs du produit
- NAD kinase (Mycobacterium tuberculosis) is fused at the N-terminus to a His-tag.
- Pureté
- >98 % (SDS-PAGE)
- Top Product
- Discover our top product NADK Protéine
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NAD Kinase (NADK) (AA 64-298) protein (His tag)
NADK Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-446) protein (Strep Tag)Crystallography grade NADK Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
NAD Kinase (NADK) (AA 1-439) protein (Strep Tag)Crystallography grade NADK Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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~1.2 U/mg protein. (Substrate: NAD (+ ATP)) ~3.8 U/mg protein. (Substrate: NAD (+ poly(P)) One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. In 10 mM TRIS at pH 8.0, containing 1 mM MgCl2, 0.5 mM EDTA, 1 mM DTT and 1 mM PMSF.
- Agent conservateur
- Dithiothreitol (DTT)
- Précaution d'utilisation
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
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Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C. - Date de péremption
- 6 months
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- Antigène
- NADK (NAD Kinase (NADK))
- Autre désignation
- NAD Kinase (NADK Produits)
- Synonymes
- 4432404C02Rik Protein, BC004012 Protein, dJ283E3.1 Protein, NAD kinase Protein, Nadk Protein, NADK Protein
- Sujet
- NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+
- Poids moléculaire
- ~33.0kDa
- UniProt
- P0A5S6
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