WARS Protein (AA 94-471) (His tag)

N° du produit ABIN3073657

Détail du produit

Antigène: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Type de proteíne: Recombinant

Attributs du protein: AA 94-471

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette WARS protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: SAKGIDYDKL IVRFGSSKID KELINRIERA TGQRPHHFLR RGIFFSHRDM NQVLDAYENK KPFYLYTGRG PSSEAMHVGH LIPFIFTKWL QDVFNVPLVI QMTDDEKYLW KDLTLDQAYS YAVENAKDII ACGFDINKTF IFSDLDYMGM SSGFYKNVVK IQKHVTFNQV KGIFGFTDSD CIGKISFPAI QAAPSFSNSF PQIFRDRTDI QCLIPCAIDQ DPYFRMTRDV APRIGYPKPA LLHSTFFPAL QGAQTKMSAS DPNSSIFLTD TAKQIKTKVN KHAFSGGRDT IEEHRQFGGN CDVDVSFMYL TFFLEDDDKL EQIRKDYTSG AMLTGELKKA LIEVLQPLIA EHQARRKEVT DEIVKEFMTP RKLSFDFQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human WARS Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Autre désignation: WARS (WARS Produits)

Synonymes: GAMMA-2 Protein, IFI53 Protein, IFP53 Protein, zgc:73274 Protein, wars Protein, MGC53671 Protein, WARS Protein, TrpRS Protein, WRS Protein, 85D-WRS Protein, CG9735 Protein, Dmel\\CG9735 Protein, WRS-85D Protein, l(3)03559 Protein, l(3)03560 Protein, l(3)3559 Protein, gamma-2 Protein, ifi53 Protein, ifp53 Protein, GB14934 Protein, ECK3371 Protein, JW3347 Protein, tryptophanyl-tRNA synthetase Protein, tryptophanyl-tRNA synthetase S homeolog Protein, Tryptophanyl-tRNA synthetase Protein, tryptophanyl-tRNA synthetase L homeolog Protein, tryptophan--tRNA ligase, cytoplasmic Protein, WARS Protein, wars Protein, wars.S Protein, Wars Protein, TrpRS Protein, wars.L Protein, LOC727582 Protein, trpS Protein, trpS2 Protein

Sujet: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. {ECO:0000269|PubMed:11773625, ECO:0000269|PubMed:11773626, ECO:0000269|PubMed:1373391, ECO:0000269|PubMed:14630953}.

Poids moléculaire: 44.3 kDa Including tag.

UniProt: P23381