GTF2E1 Protein (AA 2-439) (His tag)

N° du produit ABIN3073755

Détail du produit

Antigène: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-439

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette GTF2E1 protéine est marqué à la His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Séquence: ADPDVLTEVP AALKRLAKYV IRGFYGIEHA LALDILIRNS CVKEEDMLEL LKFDRKQLRS VLNNLKGDKF IKCRMRVETA ADGKTTRHNY YFINYRTLVN VVKYKLDHMR RRIETDERDS TNRASFKCPV CSSTFTDLEA NQLFDPMTGT FRCTFCHTEV EEDESAMPKK DARTLLARFN EQIEPIYALL RETEDVNLAY EILEPEPTEI PALKQSKDHA ATTAGAASLA GGHHREAWAT KGPSYEDLYT QNVVINMDDQ EDLHRASLEG KSAKERPIWL RESTVQGAYG SEDMKEGGID MDAFQEREEG HAGPDDNEEV MRALLIHEKK TSSAMAGSVG AAAPVTAANG SDSESETSES DDDSPPRPAA VAVHKREEDE EEDDEFEEVA DDPIVMVAGR PFSYSEVSQR PELVAQMTPE EKEAYIAMGQ RMFEDLFE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GTF2E1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Autre désignation: GTF2E1 (GTF2E1 Produits)

Synonymes: 2610024P03Rik Protein, 56kDa Protein, AI303800 Protein, FE Protein, TFIIE-A Protein, DDBDRAFT_0168201 Protein, DDBDRAFT_0168202 Protein, DDBDRAFT_0231096 Protein, DDB_0168201 Protein, DDB_0168202 Protein, DDB_0231096 Protein, DDBDRAFT_0217176 Protein, DDBDRAFT_0217177 Protein, DDBDRAFT_0266401 Protein, DDB_0217176 Protein, DDB_0217177 Protein, DDB_0266401 Protein, TF2E1 Protein, si:ch211-194d6.2 Protein, gtf2e1 Protein, tfiiealpha Protein, general transcription factor II E, polypeptide 1 (alpha subunit) Protein, general transcription factor IIE subunit 1 Protein, transcription factor IIE Protein, general transcription factor IIE subunit 2 Protein, general transcription factor IIE, polypeptide 2, beta 34kDa Protein, general transcription factor IIE, polypeptide 1, alpha Protein, general transcription factor IIE subunit 1 L homeolog Protein, Gtf2e1 Protein, gtf2e1-1 Protein, gtf2e1-2 Protein, GTF2E2 Protein, GTF2E1 Protein, gtf2e1 Protein, gtf2e1.L Protein

Sujet: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Poids moléculaire: 50.3 kDa Including tag.

UniProt: P29083