Transferrin Protein (TF) (AA 20-698) (His tag)

N° du produit ABIN3074468

Détail du produit

Antigène: Transferrin (TF)

Type de proteíne: Recombinant

Attributs du protein: AA 20-698

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette Transferrin protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: VPDKTVRWCA VSEHEATKCQ SFRDHMKSVI PSDGPSVACV KKASYLDCIR AIAANEADAV TLDAGLVYDA YLAPNNLKPV VAEFYGSKED PQTFYYAVAV VKKDSGFQMN QLRGKKSCHT GLGRSAGWNI PIGLLYCDLP EPRKPLEKAV ANFFSGSCAP CADGTDFPQL CQLCPGCGCS TLNQYFGYSG AFKCLKDGAG DVAFVKHSTI FENLANKADR DQYELLCLDN TRKPVDEYKD CHLAQVPSHT VVARSMGGKE DLIWELLNQA QEHFGKDKSK EFQLFSSPHG KDLLFKDSAH GFLKVPPRMD AKMYLGYEYV TAIRNLREGT CPEAPTDECK PVKWCALSHH ERLKCDEWSV NSVGKIECVS AETTEDCIAK IMNGEADAMS LDGGFVYIAG KCGLVPVLAE NYNKSDNCED TPEAGYFAIA VVKKSASDLT WDNLKGKKSC HTAVGRTAGW NIPMGLLYNK INHCRFDEFF SEGCAPGSKK DSSLCKLCMG SGLNLCEPNN KEGYYGYTGA FRCLVEKGDV AFVKHQTVPQ NTGGKNPDPW AKNLNEKDYE LLCLDGTRKP VEEYANCHLA RAPNHAVVTR KDKEACVHKI LRQQQHLFGS NVTDCSGNFC LFRSETKDLL FRDDTVCLAK LHDRNTYEKY LGEEYVKAVG NLRKCSTSSL LEACTFRRP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TF Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Transferrin (TF)

Autre désignation: Transferrin (TF Produits)

Synonymes: ltf Protein, pro1557 Protein, pro2086 Protein, mgc107777 Protein, LOC692564 Protein, TF Protein, LOC100144362 Protein, tf Protein, LTF Protein, TFEW Protein, conalbumin Protein, tf-b Protein, MGC64306 Protein, PRO1557 Protein, PRO2086 Protein, TFQTL1 Protein, AI266983 Protein, Cd176 Protein, HP Protein, Tf Protein, Tfn Protein, hpx Protein, Trf Protein, cb285 Protein, gavi Protein, id:ibd3238 Protein, id:ibd3525 Protein, sb:cb285 Protein, wu:fb57g06 Protein, wu:fb62h02 Protein, wu:fb63h10 Protein, wu:fb64h10 Protein, zgc:112154 Protein, 143958_at Protein, CG6186 Protein, Dmel\\CG6186 Protein, TSF1 Protein, anon-EST:Posey265 Protein, tsf1 Protein, Pro-TRH Protein, IL-5 Protein, STF I Protein, TRF1 Protein, sTF1 Protein, sTf Protein, tf1 Protein, transferrin Protein, serotransferrin Protein, transferrin (ovotransferrin) Protein, transferrin L homeolog Protein, melanotransferrin Protein, transferrin-a Protein, Transferrin 1 Protein, thyrotropin releasing hormone Protein, interleukin 5 Protein, TF Protein, LOC477072 Protein, tf Protein, Tf Protein, LOC100144362 Protein, tf.L Protein, LOC5575625 Protein, Trf Protein, tfa Protein, Tsf1 Protein, TRH Protein, IL5 Protein, LOC101085148 Protein, LOC100726872 Protein, trf Protein

Sujet: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

Poids moléculaire: 76.1 kDa Including tag.

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis