VPS45 Protein (AA 1-570) (Strep Tag)

N° du produit ABIN3075370

Détail du produit

Antigène: VPS45 (Vacuolar Protein Sorting 45 (VPS45))

Type de proteíne: Recombinant

Attributs du protein: AA 1-570

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette VPS45 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR EIMKHLKAIC FLRPTKENVD YIIQELRRPK YTIYFIYFSN VISKSDVKSL AEADEQEVVA EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS SEAAKRLAEC VKQVITKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKKPK EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ NDHSSALQNI KRLLQNPKVT EFDAARLVML YALHYERHSS NSLPGLMMDL RNKGVSEKYR KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG RLKENLYPYL GPSTLRDRPQ DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTVHNT KSFLEEVLAS GLHSRSKESS QVTSRSASRR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: VPS45 (Vacuolar Protein Sorting 45 (VPS45))

Autre désignation: VPS45 (VPS45 Produits)

Synonymes: H1 Protein, H1VPS45 Protein, SCN5 Protein, VPS45A Protein, VPS45B Protein, VPS54A Protein, VSP45 Protein, VSP45A Protein, Vsp45a Protein, AI462172 Protein, AW554165 Protein, mVps45 Protein, vps45 Protein, CG8228 Protein, DmVps45 Protein, Dmel\\CG8228 Protein, MENE (3R)-C Protein, MENE(3R)-C Protein, VPS45 Protein, Vps45p Protein, dVps45 Protein, ATVPS45 Protein, T14N5.2 Protein, T14N5_2 Protein, vacuolar protein sorting 45 Protein, si:dkey-200h23.3 Protein, vacuolar protein sorting 45 homolog Protein, vacuolar protein sorting 45 Protein, vacuolar protein sorting-associated protein 45 Protein, vacuolar protein sorting 45 homolog (S. cerevisiae) Protein, Vacuolar protein sorting 45 Protein, vacuolar protein sorting 45 homolog S homeolog Protein, SM/Sec1-family protein Protein, VPS45 Protein, Vps45 Protein, vps45 Protein, Tb10.6k15.3990 Protein, NCU06192 Protein, ATEG_06962 Protein, ANI_1_1110134 Protein, PVX_002720 Protein, AOR_1_98114 Protein, EHI_154290 Protein, PTRG_01192 Protein, UREG_03096 Protein, BDBG_07718 Protein, MCYG_05416 Protein, CC1G_15034 Protein, LOAG_17963 Protein, MGYG_06469 Protein, vps45.S Protein, LOC587303 Protein, TERG_06599 Protein

Sujet: Vacuolar protein sorting-associated protein 45 (h-VPS45) (hlVps45),FUNCTION: May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.

Poids moléculaire: 65.1 kDa

UniProt: Q9NRW7

Pathways: Transition Metal Ion Homeostasis, Synaptic Vesicle Exocytosis