RP2 Protein (AA 2-350) (His tag)

N° du produit ABIN3075514

Détail du produit

Antigène: RP2 (Retinitis Pigmentosa 2 (X-Linked Recessive) (RP2))

Type de proteíne: Recombinant

Attributs du protein: AA 2-350

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette RP2 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: GCFFSKRRKA DKESRPENEE ERPKQYSWDQ REKVDPKDYM FSGLKDETVG RLPGTVAGQQ FLIQDCENCN IYIFDHSATV TIDDCTNCII FLGPVKGSVF FRNCRDCKCT LACQQFRVRD CRKLEVFLCC ATQPIIESSS NIKFGCFQWY YPELAFQFKD AGLSIFNNTW SNIHDFTPVS GELNWSLLPE DAVVQDYVPI PTTEELKAVR VSTEANRSIV PISRGQRQKS SDESCLVVLF AGDYTIANAR KLIDEMVGKG FFLVQTKEVS MKAEDAQRVF REKAPDFLPL LNKGPVIALE FNGDGAVEVC QLIVNEIFNG TKMFVSESKE TASGDVDSFY NFADIQMGI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RP2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RP2 (Retinitis Pigmentosa 2 (X-Linked Recessive) (RP2))

Autre désignation: RP2 (RP2 Produits)

Synonymes: Rp2h Protein, RGD1565124 Protein, RP2 Protein, wu:fj10e02 Protein, wu:fm72d05 Protein, zfrp2 Protein, zgc:55632 Protein, DELXp11.3 Protein, NM23-H10 Protein, NME10 Protein, TBCCD2 Protein, XRP2 Protein, AI662636 Protein, Rp2 Protein, RP2, ARL3 GTPase activating protein Protein, XRP2 protein Protein, XRP2-like protein Protein, retinitis pigmentosa 2 (X-linked recessive) Protein, RP2, ARL3 GTPase activating protein S homeolog Protein, retinitis pigmentosa 2 homolog Protein, Rp2 Protein, RP2 Protein, Bm1_36735 Protein, PITG_13367 Protein, LOAG_11813 Protein, rp2 Protein, rp2.S Protein

Sujet: Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins. {ECO:0000269|PubMed:11847227, ECO:0000269|PubMed:18376416, ECO:0000269|PubMed:20106869, ECO:0000269|PubMed:22085962}.

Poids moléculaire: 40.5 kDa Including tag.

UniProt: O75695

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process