WD Repeat Domain 20 (WDR20) (AA 2-569) protein (His tag,GST tag)

Détails pour le produit réf. ABIN3075585
Nom de la protéine
  • DMR
  • 2310040A13Rik
  • AI549910
  • WDR20
  • Wdr20
  • wdr20
  • WD repeat domain 20
  • WD repeat domain 20a
  • WD repeat domain 20 S homeolog
  • WD repeat domain 20b
  • WDR20
  • Wdr20
  • wdr20
  • wdr20a
  • wdr20.S
  • wdr20b
Attributs du protein
AA 2-569
1
1
Origine
Humain
2
Source
Escherichia coli (E. coli)
1
1
Type de proteíne
Recombinant
Purification/Conjugué
His tag,GST tag
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Séquence MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSATEG GGKEMNEIKT QFTTREGLYK LLPHSEYSRP NRVPFNSQGS NPVRVSFVNL NDQSGNGDRL CFNVGRELYF YIYKGVRKAA DLSKPIDKRI YKGTQPTCHD FNHLTATAES VSLLVGFSAG QVQLIDPIKK ETSKLFNEER LIDKSRVTCV KWVPGSESLF LVAHSSGNMY LYNVEHTCGT TAPHYQLLKQ GESFAVHTCK SKSTRNPLLK WTVGEGALNE FAFSPDGKFL ACVSQDGFLR VFNFDSVELH GTMKSYFGGL LCVCWSPDGK YIVTGGEDDL VTVWSFVDCR VIARGHGHKS WVSVVAFDPY TTSVEEGDPM EFSGSDEDFQ DLLHFGRDRA NSTQSRLSKR NSTDSRPVSV TYRFGSVGQD TQLCLWDLTE DILFPHQPLS RARTHTNVMN ATSPPAGSNG NSVTTPGNSV PPPLPRSNSL PHSAVSNAGS KSSVMDGAIA SGVSKFATLS LHDRKERHHE KDHKRNHSMG HISSKSSDKL NLVTKTKTDP AKTLGTPLCP RMEDVPLLEP LICKKIAHER LTVLIFLEDC IVTACQEGFI CTWGRPGKVV SFNPHHHHHH
Sequence including N-terminal GST tag and C-terminal His-tag.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human WDR20 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
  • Tag Location: N-terminal GST tag, C-terminal His Tag
Purification The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Endotoxin has not been removed. Please contact us if you require endotoxin removal.
Classe de qualité Crystallography grade
Nom de la protéine
Poids moléculaire 63.7 kDa Including tag.
UniProt Q8TBZ3
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Commentaires

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
 image for WD Repeat Domain 20 (WDR20) (AA 2-569) protein (His tag,GST tag) (ABIN3075585) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
Western Blotting (WB) image for WD Repeat Domain 20 (WDR20) (AA 2-569) protein (His tag,GST tag) (ABIN3075585) WD Repeat Domain 20 (WDR20) (AA 2-569) protein (His tag,GST tag) (Image 2)
Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC) image for WD Repeat Domain 20 (WDR20) (AA 2-569) protein (His tag,GST tag) (ABIN3075585) WD Repeat Domain 20 (WDR20) (AA 2-569) protein (His tag,GST tag) (Image 3)
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