WDR20 Protein (AA 2-569) (His tag,GST tag)

N° du produit ABIN3075585

Détail du produit

Antigène: WDR20 (WD Repeat Domain 20 (WDR20))

Type de proteíne: Recombinant

Attributs du protein: AA 2-569

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette WDR20 protéine est marqué à la His tag,GST tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSATEG GGKEMNEIKT QFTTREGLYK LLPHSEYSRP NRVPFNSQGS NPVRVSFVNL NDQSGNGDRL CFNVGRELYF YIYKGVRKAA DLSKPIDKRI YKGTQPTCHD FNHLTATAES VSLLVGFSAG QVQLIDPIKK ETSKLFNEER LIDKSRVTCV KWVPGSESLF LVAHSSGNMY LYNVEHTCGT TAPHYQLLKQ GESFAVHTCK SKSTRNPLLK WTVGEGALNE FAFSPDGKFL ACVSQDGFLR VFNFDSVELH GTMKSYFGGL LCVCWSPDGK YIVTGGEDDL VTVWSFVDCR VIARGHGHKS WVSVVAFDPY TTSVEEGDPM EFSGSDEDFQ DLLHFGRDRA NSTQSRLSKR NSTDSRPVSV TYRFGSVGQD TQLCLWDLTE DILFPHQPLS RARTHTNVMN ATSPPAGSNG NSVTTPGNSV PPPLPRSNSL PHSAVSNAGS KSSVMDGAIA SGVSKFATLS LHDRKERHHE KDHKRNHSMG HISSKSSDKL NLVTKTKTDP AKTLGTPLCP RMEDVPLLEP LICKKIAHER LTVLIFLEDC IVTACQEGFI CTWGRPGKVV SFNPHHHHHH
Sequence including N-terminal GST tag and C-terminal His-tag.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human WDR20 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).
• Tag Location: N-terminal GST tag, C-terminal His Tag

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: WDR20 (WD Repeat Domain 20 (WDR20))

Autre désignation: WDR20 (WDR20 Produits)

Synonymes: DMR Protein, 2310040A13Rik Protein, AI549910 Protein, WDR20 Protein, Wdr20 Protein, wdr20 Protein, WD repeat domain 20 Protein, WD repeat domain 20a Protein, WD repeat domain 20 S homeolog Protein, WD repeat domain 20b Protein, WDR20 Protein, Wdr20 Protein, wdr20 Protein, wdr20a Protein, wdr20.S Protein, wdr20b Protein

Poids moléculaire: 63.7 kDa Including tag.

UniProt: Q8TBZ3