FBXL17 Protein (AA 1-701) (Strep Tag)

N° du produit ABIN3080464

Détail du produit

Antigène: FBXL17 (F-Box and Leucine-Rich Repeat Protein 17 (FBXL17))

Type de proteíne: Recombinant

Attributs du protein: AA 1-701

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette FBXL17 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MGHLLSKEPR NRPSQKRPRC CSWCRRRRPL LRLPRRTPAK VPPQPAAPRS RDCFFRGPCM LCFIVHSPGA PAPAGPEEEP PLSPPPRDGA YAAASSSQHL ARRYAALAAE DCAAAARRFL LSSAAAAAAA AASASSPASC CKELGLAAAA AWEQQGRSLF LASLGPVRFL GPPAAVQLFR GPTPSPAELP TPPEMVCKRK GAGVPACTPC KQPRCGGGGC GGGGGGGGGG GPAGGGASPP RPPDAGCCQA PEQPPQPLCP PPSSPTSEGA PTEAGGDAVR AGGTAPLSAQ QQHECGDADC RESPENPCDC HREPPPETPD INQLPPSILL KIFSNLSLDE RCLSASLVCK YWRDLCLDFQ FWKQLDLSSR QQVTDELLEK IASRSQNIIE INISDCRSMS DNGVCVLAFK CPGLLRYTAY RCKQLSDTSI IAVASHCPLL QKVHVGNQDK LTDEGLKQLG SKCRELKDIH FGQCYKISDE GMIVIAKGCL KLQRIYMQEN KLVTDQSVKA FAEHCPELQY VGFMGCSVTS KGVIHLTKLR NLSSLDLRHI TELDNETVME IVKRCKNLSS LNLCLNWIIN DRCVEVIAKE GQNLKELYLV SCKITDYALI AIGRYSMTIE TVDVGWCKEI TDQGATLIAQ SSKSLRYLGL MRCDKVNEVT VEQLVQQYPH ITFSTVLQDC KRTLERAYQM GWTPNMSAAS S
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: FBXL17 (F-Box and Leucine-Rich Repeat Protein 17 (FBXL17))

Autre désignation: FBXL17 (FBXL17 Produits)

Synonymes: FBXO13 Protein, Fbl17 Protein, Fbx13 Protein, 6330576B01Rik Protein, AI452053 Protein, BB073797 Protein, C130023C01Rik Protein, Fbxo13 Protein, FBXL17 Protein, F4I10.90 Protein, F4I10_90 Protein, F-box and leucine rich repeat protein 17 Protein, F-box and leucine-rich repeat protein 17 Protein, F-box and leucine-rich repeat protein 17 L homeolog Protein, RNI-like superfamily protein Protein, F-box family protein Protein, F-box/LRR-repeat protein 17 Protein, FBXL17 Protein, Fbxl17 Protein, fbxl17.L Protein, FBL17 Protein, AT4G33160 Protein, LOC100726896 Protein

Sujet: F-box/LRR-repeat protein 17 (F-box and leucine-rich repeat protein 17) (F-box only protein 13),FUNCTION: Substrate-recognition component of the SCF(FBXL17) E3 ubiquitin ligase complex, a key component of a quality control pathway required to ensure functional dimerization of BTB domain-containing proteins (dimerization quality control, DQC) (PubMed:30190310). FBXL17 specifically recognizes and binds a conserved degron of non-consecutive residues present at the interface of BTB dimers of aberrant composition: aberrant BTB dimer are then ubiquitinated by the SCF(FBXL17) complex and degraded by the proteasome (PubMed:30190310). The ability of the SCF(FBXL17) complex to eliminate compromised BTB dimers is required for the differentiation and survival of neural crest and neuronal cells (By similarity). The SCF(FBXL17) complex mediates ubiquitination and degradation of BACH1 (PubMed:24035498, PubMed:30190310). The SCF(FBXL17) complex is also involved in the regulation of the hedgehog/smoothened (Hh) signaling pathway by mediating the ubiquitination and degradation of SUFU, allowing the release of GLI1 from SUFU for proper Hh signal transduction (PubMed:27234298). The SCF(FBXL17) complex mediates ubiquitination and degradation of PRMT1 (By similarity). {ECO:0000250|UniProtKB:B1H1X1, ECO:0000250|UniProtKB:Q9QZN1, ECO:0000269|PubMed:24035498, ECO:0000269|PubMed:27234298, ECO:0000269|PubMed:30190310}.

Poids moléculaire: 75.7 kDa

UniProt: Q9UF56