EIF4E Protein (AA 2-217) (His tag)

N° du produit ABIN3081971

Détail du produit

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Type de proteíne: Recombinant

Attributs du protein: AA 2-217

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette EIF4E protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: ATVEPETTPT PNPPTTEEEK TESNQEVANP EHYIKHPLQN RWALWFFKND KSKTWQANLR LISKFDTVED FWALYNHIQL SSNLMPGCDY SLFKDGIEPM WEDEKNKRGG RWLITLNKQQ RRSDLDRFWL ETLLCLIGES FDDYSDDVCG AVVNVRAKGD KIAIWTTECE NREAVTHIGR VYKERLGLPP KIVIGYQSHA DTATKSGSTT KNRFVV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EIF4E Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Autre désignation: EIF4E (EIF4E Produits)

Synonymes: AUTS19 Protein, CBP Protein, EIF4E1 Protein, EIF4EL1 Protein, EIF4F Protein, eif4e-1 Protein, zgc:86680 Protein, EG668879 Protein, Eif4e-ps Protein, If4e Protein, eIF-4E Protein, 0260/09 Protein, 0587/11 Protein, 0589/11 Protein, 0919/12 Protein, 1004/13 Protein, 2 Protein, 7238 Protein, CG4035 Protein, CT13384 Protein, CT39424 Protein, CT39426 Protein, D-eIF4E Protein, Dmel\\CG4035 Protein, Eif4E Protein, Eif4e Protein, IF4E Protein, dEif4e Protein, deIF-4E Protein, deIF4E Protein, eIF 4E Protein, eIF-4E1 Protein, eIF-4E2 Protein, eIF-4EII Protein, eIF-4e Protein, eIF4E Protein, eIF4E-1 Protein, eIF4E-1/2 Protein, eIF4E-2 Protein, eIF4E1 Protein, eIF4EI Protein, eIF4e Protein, eif-4E Protein, eif-4e Protein, eif4e Protein, l(3)07238 Protein, l(3)67Af Protein, l(3)S025007 Protein, l(3)S026009 Protein, l(3)S058711 Protein, l(3)S091912 Protein, cbp Protein, eif4f Protein, eif4e1 Protein, eif4el1 Protein, EIF-4E Protein, zgc:101581 Protein, eukaryotic translation initiation factor 4E Protein, eukaryotic translation initiation factor 4ea Protein, Eukaryotic initiation factor 4E Protein, eucaryotic initiation factor 6 Protein, eukaryotic translation initiation factor 4E L homeolog Protein, eukaryotic translation initiation factor 4E family member 1B S homeolog Protein, translation initiation factor eIF4E Protein, eukaryotic translation initiation factor 4eb Protein, EIF4E Protein, eif4ea Protein, Eif4e Protein, eIF-4E Protein, eif6 Protein, eif4e Protein, eif4e.L Protein, eif4e1b.S Protein, LOC100533326 Protein, eif4eb Protein

Sujet: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. {ECO:0000269|PubMed:16271312, ECO:0000269|PubMed:22578813}.

Poids moléculaire: 25.9 kDa Including tag.

UniProt: P06730

Pathways: BCR Signaling