Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 Regulator 2 (SLC9A3R2) (AA 1-337) protein (His tag)

Détails pour le produit réf. ABIN3084428
Nom de la protéine
  • E3KARP
  • NHE3RF2
  • NHERF-2
  • NHERF2
  • OCTS2
  • SIP-1
  • SIP1
  • TKA-1
  • 0610011L07Rik
  • 1200011K07Rik
  • 2010007A20Rik
  • E3karp
  • Octs2
  • Sip-1
  • Sip1
  • Sryip1
  • Tka-1
  • nherf-2
  • nherf2
  • slc9a3r2
  • SLC9A3 regulator 2
  • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
  • SLC9A3 regulator 2 S homeolog
  • SLC9A3R2
  • Slc9a3r2
  • slc9a3r2.S
Attributs du protein
AA 1-337
2
1
1
1
Origine
Humain
2
1
1
1
Source
Escherichia coli (E. coli)
2
2
1
Type de proteíne
Recombinant
Purification/Conjugué
His tag
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Séquence MAAPEPLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTRLLVVDQ ETDEELRRRQ LTCTEEMAQR GLPPAHDPWE PKPDWAHTGS HSSEAGKKDV SGPLRELRPR LCHLRKGPQG YGFNLHSDKS RPGQYIRSVD PGSPAARSGL RAQDRLIEVN GQNVEGLRHA EVVASIKARE DEARLLVVDP ETDEHFKRLR VTPTEEHVEG PLPSPVTNGT SPAQLNGGSA CSSRSDLPGS DKDTEDGSAW KQDPFQESGL HLSPTAAEAK EKARAMRVNK RAPQMDWNRK REIFSNF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human SLC9A3R2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Purification Two step purification of proteins expressed in bacterial culture:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Endotoxin has not been removed. Please contact us if you require endotoxin removal.
Classe de qualité Crystallography grade
Nom de la protéine
Sujet Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3 (PubMed:18829453). May also act as scaffold protein in the nucleus. {ECO:0000269|PubMed:10455146, ECO:0000269|PubMed:18829453, ECO:0000269|PubMed:9096337}.
Poids moléculaire 38.4 kDa Including tag.
UniProt Q15599
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Commentaires

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
 image for Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 Regulator 2 (SLC9A3R2) (AA 1-337) protein (His tag) (ABIN3084428) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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