Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag)

Détails pour le produit réf. ABIN3088543, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • ACHRE
  • CMS1D
  • CMS1E
  • CMS2A
  • FCCMS
  • SCCMS
  • AChrepsilon
  • Acre
  • nAChRE
  • im:7154697
  • zgc:101010
  • CHRNE
  • cholinergic receptor nicotinic epsilon subunit
  • cholinergic receptor, nicotinic, epsilon polypeptide
  • cholinergic receptor, nicotinic epsilon L homeolog
  • cholinergic receptor, nicotinic, epsilon
  • CHRNE
  • Chrne
  • chrne.L
  • chrne
Attributs du protein
AA 21-239
2
2
2
1
1
Origine
Humain
5
3
Source
Escherichia coli (E. coli)
4
3
1
Type de proteíne
Recombinant
Purification/Conjugué
Cette CHRNE protéine est marqué à la His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Fournisseur
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N° du produit (Fournisseur)
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Séquence MHHHHHH KNEELRLYHH LFNNYDPGSR PVREPEDTVT ISLKVTLTNL ISLNEKEETL TTSVWIGIDW QDYRLNYSKD DFGGIETLRV PSELVWLPEI VLENNIDGQF GVAYDANVLV YEGGSVTWLP PAIYRSVCAV EVTYFPFDWQ NCSLIFRSQT YNAEEVEFTF AVDNDGKTIN KIDIDTEAYT ENGEWAIDFC PGVIRRHHGG ATDGPGETDV IYSLIIRRK
Sequence includes N-terminal His-tag
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Endotoxin has not been removed. Please contact us if you require endotoxin removal.
Classe de qualité Crystallography grade
Sujet After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Poids moléculaire 26.0 kDa Including tag
UniProt Q04844
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Commentaires

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer In solution (30 mM Hepes, pH 8.0, 100 mM NaCl, Laurylsarcosine)
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
 image for Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (ABIN3088543) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
 image for Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (ABIN3088543) Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (Image 2)
 image for Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (ABIN3088543) Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (Image 3)
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