C14orf45 Protein (AA 1-529) (Strep Tag)
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- Antigène Tous les produits C14orf45 (CCDC176)
- C14orf45 (CCDC176) (Coiled-Coil Domain Containing 176 (CCDC176))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-529
- Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette C14orf45 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MPSKGKDKKK GKSKGKDTKK LIKTDESVVD RAKANASLWE ARLEVTELSR IKYRDTSRIL AKSNEDLKKK QCKMEKDIMS VLSYLKKQDQ EKDNMIEKLK QQLNETKEKA QEEKDKLEQK YTRQINELEG QFHQKAKEIG MIHTELKAVR QFQKRKIQVE RELDDLKENL RNTERIHQET LRRLESRFFE EKHRLEQEAE KKIIMLAERA HHEAIVQLND AGRNVFKEND YLQKALAYHL KETDALQKNS QKLQESHTLL LHQKEINDLL VKEKIMQLVQ QRSQIQTLQK KVVNLETALS YMTKEFESEV LKLQQHAMIE NQAGQVEIDK LQHLLQMKDR EMNRVKKLAK NILDERTEVE RFFLDALHQV KQQILISRKH YKQIAQAAFN LKMRAACTGR TEYPKIRTFD GREHSTNSVN QDLLEAEKWT HIEGNVDIGD LTWEQKEKVL RLLFAKMNGC PSRKYNQSSR PPVPDYVVSD SGETKEFGDE SKLQDKIFIT QQIAISDSSG EVVLPTIPKE PQESDTGTF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Classe de qualité
- Crystallography grade
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- C14orf45 (CCDC176) (Coiled-Coil Domain Containing 176 (CCDC176))
- Autre désignation
- BBOF1 (CCDC176 Produits)
- Synonymes
- C14orf45 Protein, 2900006K08Rik Protein, AI427784 Protein, c14orf45 Protein, basal body orientation factor 1 Protein, basal body orientation factor 1 L homeolog Protein, BBOF1 Protein, Bbof1 Protein, bbof1.L Protein
- Sujet
- Basal body-orientation factor 1 (Coiled-coil domain-containing protein 176),FUNCTION: Plays an essential role in sperm motility and male fertility by stabilizing the sperm flagellar axonemal structure. May be required for the stability of ODF2 and MANS1 proteins. Dispensable for the assembly and function of motile cilia. {ECO:0000250|UniProtKB:Q3V079}.
- Poids moléculaire
- 62.0 kDa
- UniProt
- Q8ND07
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