CDH18 Protein (AA 54-608) (MBP tag)

N° du produit ABIN3090475

Détail du produit

Antigène: CDH18 (Cadherin 18, Type 2 (CDH18))

Type de proteíne: Recombinant

Attributs du protein: AA 54-608

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette CDH18 protéine est marqué à la MBP tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Séquence: GWVWNQFFVL EEHMGPDPQY VGKLHSNSDK GDGSVKYILT GEGAGTIFII DDTTGDIHST KSLDREQKTH YVLHAQAIDR RTNKPLEPES EFIIKVQDIN DNAPKFTDGP YIVTVPEMSD MGTSVLQVTA TDADDPTYGN SARVVYSILQ GQPYFSVDPK TGVIRTALHN MDREAREHYS VVIQAKDMAG QVGGLSGSTT VNITLTDVND NPPRFPQKHY QLYVPESAQV GSAVGKIKAN DADTGSNADM TYSIINGDGM GIFSISTDKE TREGILSLKK PLNYEKKKSY TLNIEGANTH LDFRFSHLGP FKDATMLKII VGDVDEPPLF SMPSYLMEVY ENAKIGTVVG TVLAQDPDST NSLVRYFINY NVEDDRFFNI DANTGTIRTT KVLDREETPW YNITVTASEI DNPDLLSHVT VGIRVLDVND NPPELAREYD IIVCENSKPG QVIHTISATD KDDFANGPRF NFFLDERLPV NPNFTLKDNE DNTASILTRR RRFSRTVQDV YYLPIMISDG GIPSLSSSST LTIRVCACER DGRVRTCHAE AFLSS
Sequence without tag.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CDH18 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using MBP capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

N-terminal MBP-Tag

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in solution (20 mM Hepes, pH 7.4, 5 mM NaCl)

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CDH18 (Cadherin 18, Type 2 (CDH18))

Autre désignation: CDH18 (CDH18 Produits)

Synonymes: B230220E17Rik Protein, Cdh14l Protein, CDH14 Protein, CDH14L Protein, CDH24 Protein, cadherin 18 Protein, Cdh18 Protein, CDH18 Protein

Sujet: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Poids moléculaire: 62.6 kDa Including tag.

UniProt: Q13634

Pathways: Cell-Cell Junction Organization