Lcor Protein (AA 1-1247) (His tag)

N° du produit ABIN3091664

Détail du produit

Antigène: Lcor (Ligand Dependent Nuclear Receptor Corepressor (Lcor))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1247

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette Lcor protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: MQSSALVESL ITVKMAAENS EEGNTCIIPQ RNLFKALSEE AWNSGFMGNS SRTADKENTL QCPKTPLRQD LEANEQDARP KQENHLHSLG RNKVGYHLHP SDKGQFDHSK DGWLGPGPMP AVHKAANGHS RTKMISTSIK TARKSKRASG LRINDYDNQC DVVYISQPIT ECHFENQKSI LSSRKTARKS TRGYFFNGDC CELPTVRTLA RNLHSQEKAS CSALASEAVF TPKQTLTIPA PRHTVDVQLP REDNPEEPSK EITSHEEGGG DVSPRKEPQE PEVCPTKIKP NLSSSPRSEE TTASSLVWPL PAHLPEEDLP EGGSTVSAPT ASGMSSPEHN QPPVALLDTE EMSVPQDCHL LPSTESFSGG VSEDVISRPH SPPEIVSREE SPQCSENQSS PMGLEPPMSL GKAEDNQSIS AEVESGDTQE LNVDPLLKES STFTDENPSE TEESEAAGGI GKLEGEDGDV KCLSEKDTYD TSIDSLEENL DKKKKGKKFP EASDRCLRSQ LSDSSSADRC LRNQSSDSSS ACLEIKVPKN PSAKRSKKEG HPGGTTPKGL LPDSFHTETL EDTEKPSVNE RPSEKDAEQE GEGGGIITRQ TLKNMLDKEV KELRGEIFPS RDPITTAGQP LPGERLEIYV QSKMDEKNAH IPSESIACKR DPEQAKEEPG HIPTQHVEEA VNEVDNENTQ QKDDESDAPC SSLGLSSSGS GDAARAPKSV PRPKRLTSST YNLRHAHSLG SLDASKVTSE KEAAQVNPIM PKENGASESG DPLDEDDVDT VVDEQPKFME WCAEEENQEL IANFNAQYMK VQKGWIQLEK EGQPTPRARN KSDKLKEIWK SKKRSRKCRS SLESQKCSPV QMLFMTNFKL SNVCKWFLET TETRSLVIVK KLNTRLPGDV PPVKHPLQKY APSSLYPSSL QAERLKKHLK KFPGATPAKN NWKMQKLWAK FRENPDQVEP EDGSDVSPGP NSEDSIEEVK EDRNSHPPAN LPTPASTRIL RKYSNIRGKL RAQQRLIKNE KMECPDALAV ESKPSRKSVC INPLMSPKLA LQVDADGFPV KPKSTEGMKG RKGKQVSEIL PKAEVQSKRK RTEGSSPPDS KNKGPTVKAS KEKHADGATK TPAAKRPAAR DRSSQPPKKT SLKENKVKIP KKSAGKSCPP SRKEKENTNK RPSQSIASET LTKPAKQKGA GESSSRPQKA TNRKQSSGKT RARPSTKTPE SSAAQRKRKL KAKLDCSHSK RRRLDAK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human C10orf12 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Lcor (Ligand Dependent Nuclear Receptor Corepressor (Lcor))

Autre désignation: C10orf12 (Lcor Produits)

Synonymes: 3110023F06Rik Protein, A630025C20Rik Protein, Mlr2 Protein, mKIAA1795 Protein, MLR2 Protein, RGD1311704 Protein, wu:fd05a07 Protein, zgc:158268 Protein, mlr2 Protein, ligand dependent nuclear receptor corepressor Protein, ligand dependent nuclear receptor corepressor S homeolog Protein, Lcor Protein, LCOR Protein, lcor.S Protein, lcor Protein

Poids moléculaire: 138.2 kDa Including tag.

UniProt: Q8N655