NCAPD3 Protein (AA 1-1498) (Strep Tag)

N° du produit ABIN3091817

Détail du produit

Antigène: NCAPD3 (Non-SMC Condensin II Complex, Subunit D3 (NCAPD3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1498

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette NCAPD3 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MVALRGLGSG LQPWCPLDLR LEWVDTVWEL DFTETEPLDP SIEAEIIETG LAAFTKLYES LLPFATGEHG SMESIWTFFI ENNVSHSTLV ALFYHFVQIV HKKNVSVQYR EYGLHAAGLY FLLLEVPGSV ANQVFHPVMF DKCIQTLKKS WPQESNLNRK RKKEQPKSSQ ANPGRHRKRG KPPRREDIEM DEIIEEQEDE NICFSARDLS QIRNAIFHLL KNFLRLLPKF SLKEKPQCVQ NCIEVFVSLT NFEPVLHECH VTQARALNQA KYIPELAYYG LYLLCSPIHG EGDKVISCVF HQMLSVILML EVGEGSHRAP LAVTSQVINC RNQAVQFISA LVDELKESIF PVVRILLQHI CAKVVDKSEY RTFAAQSLVQ LLSKLPCGEY AMFIAWLYKY SRSSKIPHRV FTLDVVLALL ELPEREVDNT LSLEHQKFLK HKFLVQEIMF DRCLDKAPTV RSKALSSFAH CLELTVTSAS ESILELLINS PTFSVIESHP GTLLRNSSAF SYQRQTSNRS EPSGEINIDS SGETVGSGER CVMAMLRRRI RDEKTNVRKS ALQVLVSILK HCDVSGMKED LWILQDQCRD PAVSVRKQAL QSLTELLMAQ PRCVQIQKAW LRGVVPVVMD CESTVQEKAL EFLDQLLLQN IRHHSHFHSG DDSQVLAWAL LTLLTTESQE LSRYLNKAFH IWSKKEKFSP TFINNVISHT GTEHSAPAWM LLSKIAGSSP RLDYSRIIQS WEKISSQQNP NSNTLGHILC VIGHIAKHLP KSTRDKVTDA VKCKLNGFQW SLEVISSAVD ALQRLCRASA ETPAEEQELL TQVCGDVLST CEHRLSNIVL KENGTGNMDE DLLVKYIFTL GDIAQLCPAR VEKRIFLLIQ SVLASSADAD HSPSSQGSSE APASQPPPQV RGSVMPSVIR AHAIITLGKL CLQHEDLAKK SIPALVRELE VCEDVAVRNN VIIVMCDLCI RYTIMVDKYI PNISMCLKDS DPFIRKQTLI LLTNLLQEEF VKWKGSLFFR FVSTLIDSHP DIASFGEFCL AHLLLKRNPV MFFQHFIECI FHFNNYEKHE KYNKFPQSER EKRLFSLKGK SNKERRMKIY KFLLEHFTDE QRFNITSKIC LSILACFADG ILPLDLDASE LLSDTFEVLS SKEIKLLAMR SKPDKDLLME EDDMALANVV MQEAQKKLIS QVQKRNFIEN IIPIIISLKT VLEKNKIPAL RELMHYLREV MQDYRDELKD FFAVDKQLAS ELEYDMKKYQ EQLVQEQELA KHADVAGTAG GAEVAPVAQV ALCLETVPVP AGQENPAMSP AVSQPCTPRA SAGHVAVSSP TPETGPLQRL LPKARPMSLS TIAILNSVKK AVESKSRHRS RSLGVLPFTL NSGSPEKTCS QVSSYSLEQE SNGEIEHVTK RAISTPEKSI SDVTFGAGVS YIGTPRTPSS AKEKIEGRSQ GNDILCLSLP DKPPPQPQQW NVRSPARNKD TPACSRRSLR KTPLKTAN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NCAPD3 (Non-SMC Condensin II Complex, Subunit D3 (NCAPD3))

Autre désignation: NCAPD3 (NCAPD3 Produits)

Synonymes: NCAPD3 Protein, id:ibd2253 Protein, zgc:55549 Protein, DDBDRAFT_0218111 Protein, DDBDRAFT_0304963 Protein, DDB_0218111 Protein, DDB_0304963 Protein, CAP-D3 Protein, CAPD3 Protein, hCAP-D3 Protein, hHCP-6 Protein, hcp-6 Protein, 2810487N22Rik Protein, 4632407J06Rik Protein, AI195468 Protein, AU018739 Protein, B130055D15Rik Protein, mKIAA0056 Protein, non-SMC condensin II complex, subunit D3 Protein, non-SMC condensin II complex subunit D3 L homeolog Protein, non-SMC condensin II complex subunit D3 Protein, condensin-2 complex subunit D3 Protein, Ncapd3 Protein, ncapd3.L Protein, NCAPD3 Protein, ncapd3 Protein, DDB_G0278701 Protein

Sujet: Condensin-2 complex subunit D3 (Non-SMC condensin II complex subunit D3) (hCAP-D3),FUNCTION: Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis (PubMed:14532007). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Specifically required for decatenation of centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed:27737959). {ECO:0000269|PubMed:14532007, ECO:0000269|PubMed:27737959}.

Poids moléculaire: 168.9 kDa

UniProt: P42695