CSPP1 Protein (AA 1-1256) (Strep Tag)

N° du produit ABIN3091977

Détail du produit

Antigène: CSPP1 (Cspp1) (Centrosome and Spindle Pole Associated Protein 1 (Cspp1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1256

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CSPP1 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MLFPLQVAAV TSSVRDDPLE HCVSPRTRAR SPEICKMADN LDEFIEEQKA RLAEDKAELE SDPPYMEMKG KLSAKLSENS KILISMAKEN IPPNSQQTRG SLGIDYGLSL PLGEDYERKK HKLKEELRQD YRRYLTQGIT QGKRKKNFLS TSETDPSTLG VSLPIGERLS AKERLKLERN KEYNQFLRGK EESSEKFRQV EKSTEPKSQR NKKPIGQVKP DLTSQIQTSC ENSEGPRKDV LTPSEAYEEL LNQRRLEEDR YRQLDDEIEL RNRRIIKKAN EEVGISNLKH QRFASKAGIP DRRFHRFNED RVFDRRYHRP DQDPEVSEEM DERFRYESDF DRRLSRVYTN DRMHRNKRGN MPPMEHDGDV IEQSNIRISS AENKSAPDNE TSKSANQDTC SPFAGMLFGG EDRELIQRRK EKYRLELLEQ MAEQQRNKRR EKDLELRVAA SGAQDPEKSP DRLKQFSVAP RHFEEMIPPE RPRIAFQTPL PPLSAPSVPP IPSVHPVPSQ NEDLRSGLSS ALGEMVSPRI APLPPPPLLP PLATNYRTPY DDAYYFYGSR NTFDPSLAYY GSGMMGVQPA AYVSAPVTHQ LAQPVVNTVG QNELKITSDQ VINSGLIFED KPKPSKQSLQ SYQEALQQQI REREERRKKE REEKEEYEAK LEAEMRTYNP WGKGGGGAPL RDAKGNLITD LNRMHRQNID AYHNPDARTY EDKRAVVSLD PNLATSNAEN LEDAANKSSG HMQTQSSPFA RGNVFGEPPT ELQIKQQELY KNFLRFQIEE KKQREEAERE RLRIAEEKEE RRLAEQRARI QQEYEEEQEK KREKEEEQRL KNEEHIRLAE ERQKEAERKK KEEEEKYNLQ LQHYCERDNL IGEETKHMRQ PSPIVPALQN KIASKLQRPP SVDSIIRSFI HESSMSRAQS PPVPARKNQL RAEEEKKNVI MELSEMRKQL RSEERRLQER LLHMDSDDEI PIRKKERNPM DIFDMARHRL QAPVRRQSPK GLDAATFQNV HDFNELKDRD SETRVDLKFM YLDPPRDHHT LEIQQQALLR EQQKRLNRIK MQEGAKVDLD AIPSAKVREQ RMPRDDTSDF LKNSLLESDS AFIGAYGETY PAIEDDVLPP PSQLPSARER RRNKWKGLDI DSSRPNVAPD GLSLKSISSV NVDELRVRNE ERMRRLNEFH NKPINTDDES SLVDPDDIMK HIGDDGSNSV ATEPWLRPGT SETLKRFMAE QLNQEQQQIP GKPGTFTWQG LSTAHG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CSPP1 (Cspp1) (Centrosome and Spindle Pole Associated Protein 1 (Cspp1))

Autre désignation: CSPP1 (Cspp1 Produits)

Synonymes: RGD1307055 Protein, CSPP Protein, 2310020J12Rik Protein, 4930413O22Rik Protein, AA387550 Protein, BC055915 Protein, Cspp2 Protein, Cspp3 Protein, centrosome and spindle pole associated protein 1 Protein, centrosome and spindle pole associated protein 1 L homeolog Protein, Cspp1 Protein, CSPP1 Protein, cspp1.L Protein, cspp1 Protein

Sujet: Centrosome and spindle pole-associated protein 1,FUNCTION: May play a role in cell-cycle-dependent microtubule organization. {ECO:0000269|PubMed:16826565}.

Poids moléculaire: 145.5 kDa

UniProt: Q1MSJ5