EDC4 Protein (AA 2-1401) (His tag)

N° du produit ABIN3092281

Détail du produit

Antigène: EDC4 (Enhancer of mRNA Decapping 4 (EDC4))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1401

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette EDC4 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: ASCASIDIED ATQHLRDILK LDRPAGGPSA ESPRPSSAYN GDLNGLLVPD PLCSGDSTSA NKTGLRTMPP INLQEKQVIC LSGDDSSTCI GILAKEVEIV ASSDSSISSK ARGSNKVKIQ PVAKYDWEQK YYYGNLIAVS NSFLAYAIRA ANNGSAMVRV ISVSTSERTL LKGFTGSVAD LAFAHLNSPQ LACLDEAGNL FVWRLALVNG KIQEEILVHI RQPEGTPLNH FRRIIWCPFI PEESEDCCEE SSPTVALLHE DRAEVWDLDM LRSSHSTWPV DVSQIKQGFI VVKGHSTCLS EGALSPDGTV LATASHDGYV KFWQIYIEGQ DEPRCLHEWK PHDGRPLSCL LFCDNHKKQD PDVPFWRFLI TGADQNRELK MWCTVSWTCL QTIRFSPDIF SSVSVPPSLK VCLDLSAEYL ILSDVQRKVL YVMELLQNQE EGHACFSSIS EFLLTHPVLS FGIQVVSRCR LRHTEVLPAE EENDSLGADG THGAGAMESA AGVLIKLFCV HTKALQDVQI RFQPQLNPDV VAPLPTHTAH EDFTFGESRP ELGSEGLGSA AHGSQPDLRR IVELPAPADF LSLSSETKPK LMTPDAFMTP SASLQQITAS PSSSSSGSSS SSSSSSSSLT AVSAMSSTSA VDPSLTRPPE ELTLSPKLQL DGSLTMSSSG SLQASPRGLL PGLLPAPADK LTPKGPGQVP TATSALSLEL QEVEPLGLPQ ASPSRTRSPD VISSASTALS QDIPEIASEA LSRGFGSSAP EGLEPDSMAS AASALHLLSP RPRPGPELGP QLGLDGGPGD GDRHNTPSLL EAALTQEAST PDSQVWPTAP DITRETCSTL AESPRNGLQE KHKSLAFHRP PYHLLQQRDS QDASAEQSDH DDEVASLASA SGGFGTKVPA PRLPAKDWKT KGSPRTSPKL KRKSKKDDGD AAMGSRLTEH QVAEPPEDWP ALIWQQQREL AELRHSQEEL LQRLCTQLEG LQSTVTGHVE RALETRHEQE QRRLERALAE GQQRGGQLQE QLTQQLSQAL SSAVAGRLER SIRDEIKKTV PPCVSRSLEP MAGQLSNSVA TKLTAVEGSM KENISKLLKS KNLTDAIARA AADTLQGPMQ AAYREAFQSV VLPAFEKSCQ AMFQQINDSF RLGTQEYLQQ LESHMKSRKA REQEAREPVL AQLRGLVSTL QSATEQMAAT VAGSVRAEVQ HQLHVAVGSL QESILAQVQR IVKGEVSVAL KEQQAAVTSS IMQAMRSAAG TPVPSAHLDC QAQQAHILQL LQQGHLNQAF QQALTAADLN LVLYVCETVD PAQVFGQPPC PLSQPVLLSL IQQLASDLGT RTDLKLSYLE EAVMHLDHSD PITRDHMGSV MAQVRQKLFQ FLQAEPHNSL GKAARRLSLM LHGLVTPSLP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EDC4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EDC4 (Enhancer of mRNA Decapping 4 (EDC4))

Autre désignation: EDC4 (EDC4 Produits)

Synonymes: VARICOSE Protein, ge-1 Protein, hedls Protein, rcd-8 Protein, GE1 Protein, Ge-1 Protein, HEDL5 Protein, HEDLS Protein, RCD-8 Protein, RCD8 Protein, BC022641 Protein, si:ch211-237c7.1 Protein, wu:fe26b05 Protein, LRRG00115 Protein, Nrn1l Protein, RGD1309136 Protein, enhancer of mRNA decapping 4 Protein, Transducin/WD40 repeat-like superfamily protein Protein, enhancer of mRNA decapping 4 L homeolog Protein, EDC4 Protein, VCS Protein, edc4 Protein, Edc4 Protein, edc4.L Protein

Sujet: In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro). {ECO:0000269|PubMed:16364915}.

Poids moléculaire: 152.5 kDa Including tag.

UniProt: Q6P2E9