KPNA1 Protein (AA 2-538) (His tag)

N° du produit ABIN3093111

Détail du produit

Antigène: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-538

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette KPNA1 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: TTPGKENFRL KSYKNKSLNP DEMRRRREEE GLQLRKQKRE EQLFKRRNVA TAEEETEEEV MSDGGFHEAQ ISNMEMAPGG VITSDMIEMI FSKSPEQQLS ATQKFRKLLS KEPNPPIDEV ISTPGVVARF VEFLKRKENC TLQFESAWVL TNIASGNSLQ TRIVIQAGAV PIFIELLSSE FEDVQEQAVW ALGNIAGDST MCRDYVLDCN ILPPLLQLFS KQNRLTMTRN AVWALSNLCR GKSPPPEFAK VSPCLNVLSW LLFVSDTDVL ADACWALSYL SDGPNDKIQA VIDAGVCRRL VELLMHNDYK VVSPALRAVG NIVTGDDIQT QVILNCSALQ SLLHLLSSPK ESIKKEACWT ISNITAGNRA QIQTVIDANI FPALISILQT AEFRTRKEAA WAITNATSGG SAEQIKYLVE LGCIKPLCDL LTVMDSKIVQ VALNGLENIL RLGEQEAKRN GTGINPYCAL IEEAYGLDKI EFLQSHENQE IYQKAFDLIE HYFGTEDEDS SIAPQVDLNQ QQYIFQQCEA PMEGFQL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human KPNA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Autre désignation: KPNA1 (KPNA1 Produits)

Synonymes: IPOA5 Protein, NPI-1 Protein, RCH2 Protein, SRP1 Protein, AW494490 Protein, NPI1 Protein, Rch2 Protein, mSRP1 Protein, si:ch211-161h7.7 Protein, karyopherin subunit alpha 1 Protein, importin alpha-1 subunit Protein, karyopherin (importin) alpha 1 Protein, karyopherin alpha 1 (importin alpha 5) Protein, KPNA1 Protein, Kpna1 Protein, EHI_124900 Protein, kpna1 Protein

Sujet: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

Poids moléculaire: 61.0 kDa Including tag.

UniProt: P52294

Pathways: M Phase, Protein targeting to Nucleus