KLHL22 Protein (AA 2-634) (His tag)

N° du produit ABIN3093464

Détail du produit

Antigène: KLHL22 (Kelch-Like 22 (KLHL22))

Type de proteíne: Recombinant

Attributs du protein: AA 2-634

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette KLHL22 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: AEEQEFTQLC KLPAQPSHPH CVNNTYRSAQ HSQALLRGLL ALRDSGILFD VVLVVEGRHI EAHRILLAAS CDYFRGMFAG GLKEMEQEEV LIHGVSYNAM CQILHFIYTS ELELSLSNVQ ETLVAACQLQ IPEIIHFCCD FLMSWVDEEN ILDVYRLAEL FDLSRLTEQL DTYILKNFVA FSRTDKYRQL PLEKVYSLLS SNRLEVSCET EVYEGALLYH YSLEQVQADQ ISLHEPPKLL ETVRFPLMEA EVLQRLHDKL DPSPLRDTVA SALMYHRNES LQPSLQSPQT ELRSDFQCVV GFGGIHSTPS TVLSDQAKYL NPLLGEWKHF TASLAPRMSN QGIAVLNNFV YLIGGDNNVQ GFRAESRCWR YDPRHNRWFQ IQSLQQEHAD LSVCVVGRYI YAVAGRDYHN DLNAVERYDP ATNSWAYVAP LKREVYAHAG ATLEGKMYIT CGRRGEDYLK ETHCYDPGSN TWHTLADGPV RRAWHGMATL LNKLYVIGGS NNDAGYRRDV HQVACYSCTS GQWSSVCPLP AGHGEPGIAV LDNRIYVLGG RSHNRGSRTG YVHIYDVEKD CWEEGPQLDN SISGLAACVL TLPRSLLLEP PRGTPDRSQA DPDFASEVMS VSDWEEFDNS SED
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human KLHL22 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: KLHL22 (Kelch-Like 22 (KLHL22))

Autre désignation: KLHL22 (KLHL22 Produits)

Synonymes: 2610318I18Rik Protein, Kelchl Protein, KELCHL Protein, RGD1306288 Protein, kelch-like 22 Protein, kelch like family member 22 Protein, kelch-like family member 22 Protein, Klhl22 Protein, KLHL22 Protein

Sujet: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. {ECO:0000269|PubMed:19995937, ECO:0000269|PubMed:23455478}.

Poids moléculaire: 72.5 kDa Including tag.

UniProt: Q53GT1