Neurexin 3 Protein (NRXN3) (AA 28-1568) (His tag)

N° du produit ABIN3094168

Détail du produit

Antigène: Neurexin 3 (NRXN3)

Type de proteíne: Recombinant

Attributs du protein: AA 28-1568

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette Neurexin 3 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: LEFMGLPNQW ARYLRWDAST RSDLSFQFKT NVSTGLLLYL DDGGVCDFLC LSLVDGRVQL RFSMDCAETA VLSNKQVNDS SWHFLMVSRD RLRTVLMLDG EGQSGELQPQ RPYMDVVSDL FLGGVPTDIR PSALTLDGVQ AMPGFKGLIL DLKYGNSEPR LLGSRGVQMD AEGPCGERPC ENGGICFLLD GHPTCDCSTT GYGGKLCSED VSQDPGLSHL MMSEQAREEN VATFRGSEYL CYDLSQNPIQ SSSDEITLSF KTWQRNGLIL HTGKSADYVN LALKDGAVSL VINLGSGAFE AIVEPVNGKF NDNAWHDVKV TRNLRQVTIS VDGILTTTGY TQEDYTMLGS DDFFYVGGSP STADLPGSPV SNNFMGCLKE VVYKNNDIRL ELSRLARIAD TKMKIYGEVV FKCENVATLD PINFETPEAY ISLPKWNTKR MGSISFDFRT TEPNGLILFT HGKPQERKDA RSQKNTKVDF FAVELLDGNL YLLLDMGSGT IKVKATQKKA NDGEWYHVDI QRDGRSGTIS VNSRRTPFTA SGESEILDLE GDMYLGGLPE NRAGLILPTE LWTAMLNYGY VGCIRDLFID GRSKNIRQLA EMQNAAGVKS SCSRMSAKQC DSYPCKNNAV CKDGWNRFIC DCTGTGYWGR TCEREASILS YDGSMYMKII MPMVMHTEAE DVSFRFMSQR AYGLLVATTS RDSADTLRLE LDGGRVKLMV NLDCIRINCN SSKGPETLYA GQKLNDNEWH TVRVVRRGKS LKLTVDDDVA EGTMVGDHTR LEFHNIETGI MTEKRYISVV PSSFIGHLQS LMFNGLLYID LCKNGDIDYC ELKARFGLRN IIADPVTFKT KSSYLSLATL QAYTSMHLFF QFKTTSPDGF ILFNSGDGND FIAVELVKGY IHYVFDLGNG PNVIKGNSDR PLNDNQWHNV VITRDNSNTH SLKVDTKVVT QVINGAKNLD LKGDLYMAGL AQGMYSNLPK LVASRDGFQG CLASVDLNGR LPDLINDALH RSGQIERGCE GPSTTCQEDS CANQGVCMQQ WEGFTCDCSM TSYSGNQCND PGATYIFGKS GGLILYTWPA NDRPSTRSDR LAVGFSTTVK DGILVRIDSA PGLGDFLQLH IEQGKIGVVF NIGTVDISIK EERTPVNDGK YHVVRFTRNG GNATLQVDNW PVNEHYPTGR QLTIFNTQAQ IAIGGKDKGR LFQGQLSGLY YDGLKVLNMA AENNPNIKIN GSVRLVGEVP SILGTTQTTS MPPEMSTTVM ETTTTMATTT TRKNRSTASI QPTSDDLVSS AECSSDDEDF VECEPSTTGG ELVIPLLVED PLATPPIATR APSITLPPTF RPLLTIIETT KDSLSMTSEA GLPCLSDQGS DGCDDDGLVI SGYGSGETFD SNLPPTDDED FYTTFSLVTD KSLSTSIFEG GYKAHAPKWE SKDFRPNKVS ETSRTTTTSL SPELIRFTAS SSSGMVPKLP AGKMNNRDLK PQPDIVLLPL PTAYELDSTK LKSPLITSPM FRNVPTANPT EPGIRRVPGA SEVIRESSST T
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NRXN3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Neurexin 3 (NRXN3)

Autre désignation: NRXN3 (NRXN3 Produits)

Synonymes: C14orf60 Protein, NRXN3 Protein, NRXNIII Protein, im:7144250 Protein, nrxn3aa Protein, nrxn3ab Protein, wu:fj54c11 Protein, neurexin 3 Protein, neurexin III Protein, neurexin-3 Protein, neurexin 3a Protein, neurexin 3b Protein, NRXN3 Protein, Nrxn3 Protein, nrxn3 Protein, LOC490812 Protein, nrxn3a Protein, nrxn3b Protein

Sujet: Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May mediate intracellular signaling.

Poids moléculaire: 170.2 kDa Including tag.

UniProt: Q9Y4C0