PELP1 Protein (AA 2-1130) (His tag)

N° du produit ABIN3094487

Détail du produit

Antigène: PELP1 (Proline-, Glutamic Acid- and Leucine-Rich Protein 1 (PELP1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1130

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette PELP1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: AAAVLSGPSA GSAAGVPGGT GGLSAVSSGP RLRLLLLESV SGLLQPRTGS AVAPVHPPNR SAPHLPGLMC LLRLHGSVGG AQNLSALGAL VSLSNARLSS IKTRFEGLCL LSLLVGESPT ELFQQHCVSW LRSIQQVLQT QDPPATMELA VAVLRDLLRY AAQLPALFRD ISMNHLPGLL TSLLGLRPEC EQSALEGMKA CMTYFPRACG SLKGKLASFF LSRVDALSPQ LQQLACECYS RLPSLGAGFS QGLKHTESWE QELHSLLASL HTLLGALYEG AETAPVQNEG PGVEMLLSSE DGDAHVLLQL RQRFSGLARC LGLMLSSEFG APVSVPVQEI LDFICRTLSV SSKNISLHGD GPLRLLLLPS IHLEALDLLS ALILACGSRL LRFGILIGRL LPQVLNSWSI GRDSLSPGQE RPYSTVRTKV YAILELWVQV CGASAGMLQG GASGEALLTH LLSDISPPAD ALKLRSPRGS PDGSLQTGKP SAPKKLKLDV GEAMAPPSHR KGDSNANSDV CAAALRGLSR TILMCGPLIK EETHRRLHDL VLPLVMGVQQ GEVLGSSPYT SSRCRRELYC LLLALLLAPS PRCPPPLACA LQAFSLGQRE DSLEVSSFCS EALVTCAALT HPRVPPLQPM GPTCPTPAPV PPPEAPSPFR APPFHPPGPM PSVGSMPSAG PMPSAGPMPS AGPVPSARPG PPTTANHLGL SVPGLVSVPP RLLPGPENHR AGSNEDPILA PSGTPPPTIP PDETFGGRVP RPAFVHYDKE EASDVEISLE SDSDDSVVIV PEGLPPLPPP PPSGATPPPI APTGPPTASP PVPAKEEPEE LPAAPGPLPP PPPPPPPVPG PVTLPPPQLV PEGTPGGGGP PALEEDLTVI NINSSDEEEE EEEEEEEEEE EEEEEEEDFE EEEEDEEEYF EEEEEEEEEF EEEFEEEEGE LEEEEEEEDE EEEEELEEVE DLEFGTAGGE VEEGAPPPPT LPPALPPPES PPKVQPEPEP EPGLLLEVEE PGTEEERGAD TAPTLAPEAL PSQGEVEREG ESPAAGPPPQ ELVEEEPSAP PTLLEEETED GSDKVQPPPE TPAEEEMETE TEAEALQEKE QDDTAAMLAD FIDCPPDDEK PPPPTEPDS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PELP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PELP1 (Proline-, Glutamic Acid- and Leucine-Rich Protein 1 (PELP1))

Autre désignation: PELP1 (PELP1 Produits)

Synonymes: MNAR Protein, P160 Protein, RGD1306320 Protein, 4930563C04Rik Protein, proline, glutamate and leucine rich protein 1 Protein, proline, glutamate and leucine rich protein 1 L homeolog Protein, proline, glutamic acid and leucine rich protein 1 Protein, PELP1 Protein, Pelp1 Protein, pelp1.L Protein

Sujet: Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex, the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. {ECO:0000269|PubMed:11481323, ECO:0000269|PubMed:12415108, ECO:0000269|PubMed:12682072, ECO:0000269|PubMed:14963108, ECO:0000269|PubMed:15374949, ECO:0000269|PubMed:15456770, ECO:0000269|PubMed:15579769, ECO:0000269|PubMed:15994929, ECO:0000269|PubMed:16140940, ECO:0000269|PubMed:16352611, ECO:0000269|PubMed:16574651, ECO:0000269|PubMed:22872859}.

Poids moléculaire: 120.5 kDa Including tag.

UniProt: Q8IZL8