RPRD2 Protein (AA 1-1461) (Strep Tag)

N° du produit ABIN3095101

Détail du produit

Antigène: RPRD2 (Regulation of Nuclear Pre-mRNA Domain Containing 2 (RPRD2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1461

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette RPRD2 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MAAGGGGGSS KASSSSASSA GALESSLDRK FQSVTNTMES IQGLSSWCIE NKKHHSTIVY HWMKWLRRSA YPHRLNLFYL ANDVIQNCKR KNAIIFRESF ADVLPEAAAL VKDPSVSKSV ERIFKIWEDR NVYPEEMIVA LREALSTTFK TQKQLKENLN KQPNKQWKKS QTSTNPKAAL KSKIVAEFRS QALIEELLLY KRSEDQIELK EKQLSTMRVD VCSTETLKCL KDKTGGKKFS KEFEEASSKL EEFVNGLDKQ VKNGPSLTEA LENAGIFYEA QYKEVKVVAN AYKTFANRVN NLKKKLDQLK STLPDPEESP VPSPSMDAPS PTGSESPFQG MGGEESQSPT MESEKSATPE PVTDNRDVED MELSDVEDDG SKIIVEDRKE KPAEKSAVST SVPTKPTENI SKASSCTPVP VTMTATPPLP KPVNTSLSPS PALALPNLAN VDLAKISSIL SSLTSVMKNT GVSPASRPSP GTPTSPSNLT SGLKTPAPAT TTSHNPLANI LSKVEITPES ILSALSKTQT QSAPALQGLS SLLQSVTGNP VPASEAASQS TSASPANTTV STIKGRNLPS SAQPFIPKSF NYSPNSSTSE VSSTSASKAS IGQSPGLPST TFKLPSNSLG FTATHNTSPA APPTEVTICQ SSEVSKPKLE SESTSPSLEM KIHNFLKGNP GFSGLNLNIP ILSSLGSSAP SESHPSDFQR GPTSTSIDNI DGTPVRDERS GTPTQDEMMD KPTSSSVDTM SLLSKIISPG SSTPSSTRSP PPGRDESYPR ELSNSVSTYR PFGLGSESPY KQPSDGMERP SSLMDSSQEK FYPDTSFQED EDYRDFEYSG PPPSAMMNLE KKPAKSILKS SKLSDTTEYQ PILSSYSHRA QEFGVKSAFP PSVRALLDSS ENCDRLSSSP GLFGAFSVRG NEPGSDRSPS PSKNDSFFTP DSNHNSLSQS TTGHLSLPQK QYPDSPHPVP HRSLFSPQNT LAAPTGHPPT SGVEKVLAST ISTTSTIEFK NMLKNASRKP SDDKHFGQAP SKGTPSDGVS LSNLTQPSLT ATDQQQQEEH YRIETRVSSS CLDLPDSTEE KGAPIETLGY HSASNRRMSG EPIQTVESIR VPGKGNRGHG REASRVGWFD LSTSGSSFDN GPSSASELAS LGGGGSGGLT GFKTAPYKER APQFQESVGS FRSNSFNSTF EHHLPPSPLE HGTPFQREPV GPSSAPPVPP KDHGGIFSRD APTHLPSVDL SNPFTKEAAL AHAAPPPPPG EHSGIPFPTP PPPPPPGEHS SSGGSGVPFS TPPPPPPPVD HSGVVPFPAP PLAEHGVAGA VAVFPKDHSS LLQGTLAEHF GVLPGPRDHG GPTQRDLNGP GLSRVRESLT LPSHSLEHLG PPHGGGGGGG SNSSSGPPLG PSHRDTISRS GIILRSPRPD FRPREPFLSR DPFHSLKRPR PPFARGPPFF APKRPFFPPR Y
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RPRD2 (Regulation of Nuclear Pre-mRNA Domain Containing 2 (RPRD2))

Autre désignation: RPRD2 (RPRD2 Produits)

Synonymes: fk52b12 Protein, rprd2 Protein, wu:fk52b12 Protein, zgc:63796 Protein, KIAA0460 Protein, 2810036A19Rik Protein, 4930535B03Rik Protein, 6720469I21Rik Protein, AL022841 Protein, AL022940 Protein, AU021304 Protein, BB077382 Protein, mKIAA0460 Protein, regulation of nuclear pre-mRNA domain containing 2a Protein, regulation of nuclear pre-mRNA domain containing 2 Protein, regulation of nuclear pre-mRNA domain containing 2b Protein, rprd2a Protein, RPRD2 Protein, rprd2b Protein, Rprd2 Protein

Sujet: Regulation of nuclear pre-mRNA domain-containing protein 2

Poids moléculaire: 156.0 kDa

UniProt: Q5VT52