SLIT3 Protein (AA 34-1523) (His tag)

N° du produit ABIN3095436

Détail du produit

Antigène: SLIT3 (Slit Homolog 3 (SLIT3))

Type de proteíne: Recombinant

Attributs du protein: AA 34-1523

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette SLIT3 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: CPTKCTCSAA SVDCHGLGLR AVPRGIPRNA ERLDLDRNNI TRITKMDFAG LKNLRVLHLE DNQVSVIERG AFQDLKQLER LRLNKNKLQV LPELLFQSTP KLTRLDLSEN QIQGIPRKAF RGITDVKNLQ LDNNHISCIE DGAFRALRDL EILTLNNNNI SRILVTSFNH MPKIRTLRLH SNHLYCDCHL AWLSDWLRQR RTVGQFTLCM APVHLRGFNV ADVQKKEYVC PAPHSEPPSC NANSISCPSP CTCSNNIVDC RGKGLMEIPA NLPEGIVEIR LEQNSIKAIP AGAFTQYKKL KRIDISKNQI SDIAPDAFQG LKSLTSLVLY GNKITEIVKG LFDGLVSLQL LLLNANKINC LRVNTFQDLQ NLNLLSLYDN KLQTISKGLF APLQSIQTLH LAQNPFVCDC HLKWLADYLQ DNPIETSGAR CSSPRRLANK RISQIKSKKF RCSGSEDYRS RFSSECFMDL VCPEKCRCEG TIVDCSNQKL VRIPSHLPEY VTDLRLNDNE VSVLEATGIF KKLPNLRKIN LSNNKIKEVR EGAFDGAASV QELMLTGNQL ETVHGRVFRG LSGLKTLMLR SNLIGCVSND TFAGLSSVRL LSLYDNRITT ITPGAFTTLV SLSTINLLSN PFNCNCHLAW LGKWLRKRRI VSGNPRCQKP FFLKEIPIQD VAIQDFTCDG NEESSCQLSP RCPEQCTCME TVVRCSNKGL RALPRGMPKD VTELYLEGNH LTAVPRELSA LRHLTLIDLS NNSISMLTNY TFSNMSHLST LILSYNRLRC IPVHAFNGLR SLRVLTLHGN DISSVPEGSF NDLTSLSHLA LGTNPLHCDC SLRWLSEWVK AGYKEPGIAR CSSPEPMADR LLLTTPTHRF QCKGPVDINI VAKCNACLSS PCKNNGTCTQ DPVELYRCAC PYSYKGKDCT VPINTCIQNP CQHGGTCHLS DSHKDGFSCS CPLGFEGQRC EINPDDCEDN DCENNATCVD GINNYVCICP PNYTGELCDE VIDHCVPELN LCQHEAKCIP LDKGFSCECV PGYSGKLCET DNDDCVAHKC RHGAQCVDTI NGYTCTCPQG FSGPFCEHPP PMVLLQTSPC DQYECQNGAQ CIVVQQEPTC RCPPGFAGPR CEKLITVNFV GKDSYVELAS AKVRPQANIS LQVATDKDNG ILLYKGDNDP LALELYQGHV RLVYDSLSSP PTTVYSVETV NDGQFHSVEL VTLNQTLNLV VDKGTPKSLG KLQKQPAVGI NSPLYLGGIP TSTGLSALRQ GTDRPLGGFH GCIHEVRINN ELQDFKALPP QSLGVSPGCK SCTVCKHGLC RSVEKDSVVC ECRPGWTGPL CDQEARDPCL GHRCHHGKCV ATGTSYMCKC AEGYGGDLCD NKNDSANACS AFKCHHGQCH ISDQGEPYCL CQPGFSGEHC QQENPCLGQV VREVIRRQKG YASCATASKV PIMECRGGCG PQCCQPTRSK RRKYVFQCTD GSSFVEEVER HLECGCLACS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SLIT3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SLIT3 (Slit Homolog 3 (SLIT3))

Autre désignation: SLIT3 (SLIT3 Produits)

Synonymes: MGC146100 Protein, zgc:111911 Protein, MEGF5 Protein, SLIL2 Protein, SLIT1 Protein, Slit-3 Protein, slit2 Protein, Slil2 Protein, Slit1 Protein, Megf5 Protein, slit guidance ligand 3 Protein, slit homolog 3 (Drosophila) Protein, Slit3 Protein, slit3 Protein, SLIT3 Protein, LOC100125612 Protein, Slit3 Protein

Sujet: May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.

Poids moléculaire: 165.6 kDa Including tag.

UniProt: O75094

Pathways: Regulation of Cell Size