SMARCD3 Protein (AA 2-483) (His tag)

N° du produit ABIN3095592

Détail du produit

Antigène: SMARCD3 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily D, Member 3 (SMARCD3))

Type de proteíne: Recombinant

Attributs du protein: AA 2-483

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SMARCD3 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: AADEVAGGAR KATKSKLFEF LVHGVRPGMP SGARMPHQGA PMGPPGSPYM GSPAVRPGLA PAGMEPARKR AAPPPGQSQA QSQGQPVPTA PARSRSAKRR KMADKILPQR IRELVPESQA YMDLLAFERK LDQTIMRKRV DIQEALKRPM KQKRKLRLYI SNTFNPAKPD AEDSDGSIAS WELRVEGKLL DDPSKQKRKF SSFFKSLVIE LDKDLYGPDN HLVEWHRTPT TQETDGFQVK RPGDLSVRCT LLLMLDYQPP QFKLDPRLAR LLGLHTQSRS AIVQALWQYV KTNRLQDSHD KEYINGDKYF QQIFDCPRLK FSEIPQRLTA LLLPPDPIVI NHVISVDPSD QKKTACYDID VEVEEPLKGQ MSSFLLSTAN QQEISALDSK IHETIESINQ LKIQRDFMLS FSRDPKGYVQ DLLRSQSRDL KVMTDVAGNP EEERRAEFYH QPWSQEAVSR YFYCKIQQRR QELEQSLVVR NT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SMARCD3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SMARCD3 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily D, Member 3 (SMARCD3))

Autre désignation: SMARCD3 (SMARCD3 Produits)

Synonymes: SMARCD3 Protein, baf60c Protein, cracd3 Protein, rsc6p Protein, BAF60C Protein, CRACD3 Protein, Rsc6p Protein, 1500001J14Rik Protein, 2210409C08Rik Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 S homeolog Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3b Protein, SMARCD3 Protein, smarcd3.S Protein, Smarcd3 Protein, smarcd3b Protein

Sujet: Plays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). {ECO:0000250, ECO:0000269|PubMed:8804307}.

Poids moléculaire: 55.8 kDa Including tag.

UniProt: Q6STE5

Pathways: Regulation of Lipid Metabolism by PPARalpha