USP40 Protein (AA 1-1235) (Strep Tag)

N° du produit ABIN3096235

Détail du produit

Antigène: USP40 (Ubiquitin Specific Peptidase 40 (USP40))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1235

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette USP40 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MFGDLFEEEY STVSNNQYGK GKKLKTKALE PPAPREFTNL SGIRNQGGTC YLNSLLQTLH FTPEFREALF SLGPEELGLF EDKDKPDAKV RIIPLQLQRL FAQLLLLDQE AASTADLTDS FGWTSNEEMR QHDVQELNRI LFSALETSLV GTSGHDLIYR LYHGTIVNQI VCKECKNVSE RQEDFLDLTV AVKNVSGLED ALWNMYVEEE VFDCDNLYHC GTCDRLVKAA KSAKLRKLPP FLTVSLLRFN FDFVKCERYK ETSCYTFPLR INLKPFCEQS ELDDLEYIYD LFSVIIHKGG CYGGHYHVYI KDVDHLGNWQ FQEEKSKPDV NLKDLQSEEE IDHPLMILKA ILLEENNLIP VDQLGQKLLK KIGISWNKKY RKQHGPLRKF LQLHSQIFLL SSDESTVRLL KNSSLQAESD FQRNDQQIFK MLPPESPGLN NSISCPHWFD INDSKVQPIR EKDIEQQFQG KESAYMLFYR KSQLQRPPEA RANPRYGVPC HLLNEMDAAN IELQTKRAEC DSANNTFELH LHLGPQYHFF NGALHPVVSQ TESVWDLTFD KRKTLGDLRQ SIFQLLEFWE GDMVLSVAKL VPAGLHIYQS LGGDELTLCE TEIADGEDIF VWNGVEVGGV HIQTGIDCEP LLLNVLHLDT SSDGEKCCQV IESPHVFPAN AEVGTVLTAL AIPAGVIFIN SAGCPGGEGW TAIPKEDMRK TFREQGLRNG SSILIQDSHD DNSLLTKEEK WVTSMNEIDW LHVKNLCQLE SEEKQVKISA TVNTMVFDIR IKAIKELKLM KELADNSCLR PIDRNGKLLC PVPDSYTLKE AELKMGSSLG LCLGKAPSSS QLFLFFAMGS DVQPGTEMEI VVEETISVRD CLKLMLKKSG LQGDAWHLRK MDWCYEAGEP LCEEDATLKE LLICSGDTLL LIEGQLPPLG FLKVPIWWYQ LQGPSGHWES HQDQTNCTSS WGRVWRATSS QGASGNEPAQ VSLLYLGDIE ISEDATLAEL KSQAMTLPPF LEFGVPSPAH LRAWTVERKR PGRLLRTDRQ PLREYKLGRR IEICLEPLQK GENLGPQDVL LRTQVRIPGE RTYAPALDLV WNAAQGGTAG SLRQRVADFY RLPVEKIEIA KYFPEKFEWL PISSWNQQIT KRKKKKKQDY LQGAPYYLKD GDTIGVKNLL IDDDDDFSTI RDDTGKEKQK QRALGRRKSQ EALHEQSSYI LSSAETPARP RAPETSLSIH VGSFR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: USP40 (Ubiquitin Specific Peptidase 40 (USP40))

Autre désignation: USP40 (USP40 Produits)

Synonymes: B230215L03Rik Protein, C730029K03 Protein, LRRGT00071 Protein, ubiquitin specific peptidase 40 Protein, TRAF3 interacting protein 1 Protein, usp40 Protein, TRAF3IP1 Protein, USP40 Protein, Usp40 Protein

Sujet: Ubiquitin carboxyl-terminal hydrolase 40 (EC 3.4.19.12) (Deubiquitinating enzyme 40) (Ubiquitin thioesterase 40) (Ubiquitin-specific-processing protease 40),FUNCTION: May be catalytically inactive.

Poids moléculaire: 140.1 kDa

UniProt: Q9NVE5