WDR33 Protein (AA 2-1336) (His tag)

N° du produit ABIN3096390

Détail du produit

Antigène: WDR33 (WD Repeat Domain 33 (WDR33))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1336

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette WDR33 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: ATEIGSPPRF FHMPRFQHQA PRQLFYKRPD FAQQQAMQQL TFDGKRMRKA VNRKTIDYNP SVIKYLENRI WQRDQRDMRA IQPDAGYYND LVPPIGMLNN PMNAVTTKFV RTSTNKVKCP VFVVRWTPEG RRLVTGASSG EFTLWNGLTF NFETILQAHD SPVRAMTWSH NDMWMLTADH GGYVKYWQSN MNNVKMFQAH KEAIREASFS PTDNKFATCS DDGTVRIWDF LRCHEERILR GHGADVKCVD WHPTKGLVVS GSKDSQQPIK FWDPKTGQSL ATLHAHKNTV MEVKLNLNGN WLLTASRDHL CKLFDIRNLK EELQVFRGHK KEATAVAWHP VHEGLFASGG SDGSLLFWHV GVEKEVGGME MAHEGMIWSL AWHPLGHILC SGSNDHTSKF WTRNRPGDKM RDRYNLNLLP GMSEDGVEYD DLEPNSLAVI PGMGIPEQLK LAMEQEQMGK DESNEIEMTI PGLDWGMEEV MQKDQKKVPQ KKVPYAKPIP AQFQQAWMQN KVPIPAPNEV LNDRKEDIKL EEKKKTQAEI EQEMATLQYT NPQLLEQLKI ERLAQKQVEQ IQPPPSSGTP LLGPQPFPGQ GPMSQIPQGF QQPHPSQQMP MNMAQMGPPG PQGQFRPPGP QGQMGPQGPP LHQGGGGPQG FMGPQGPQGP PQGLPRPQDM HGPQGMQRHP GPHGPLGPQG PPGPQGSSGP QGHMGPQGPP GPQGHIGPQG PPGPQGHLGP QGPPGTQGMQ GPPGPRGMQG PPHPHGIQGG PGSQGIQGPV SQGPLMGLNP RGMQGPPGPR ENQGPAPQGM IMGHPPQEMR GPHPPGGLLG HGPQEMRGPQ EIRGMQGPPP QGSMLGPPQE LRGPPGSQSQ QGPPQGSLGP PPQGGMQGPP GPQGQQNPAR GPHPSQGPIP FQQQKTPLLG DGPRAPFNQE GQSTGPPPLI PGLGQQGAQG RIPPLNPGQG PGPNKGDSRG PPNHHMGPMS ERRHEQSGGP EHGPERGPFR GGQDCRGPPD RRGPHPDFPD DFSRPDDFHP DKRFGHRLRE FEGRGGPLPQ EEKWRRGGPG PPFPPDHREF SEGDGRGAAR GPPGAWEGRR PGDERFPRDP EDPRFRGRRE ESFRRGAPPR HEGRAPPRGR DGFPGPEDFG PEENFDASEE AARGRDLRGR GRGTPRGGRK GLLPTPDEFP RFEGGRKPDS WDGNREPGPG HEHFRDTPRP DHPPHDGHSP ASRERSSSLQ GMDMASLPPR KRPWHDGPGT SEHREMEAPG GPSEDRGGKG RGGPGPAQRV PKSGRSSSLD GEHHDGYHRD EPFGGPPGSG TPSRGGRSGS NWGRGSNMNS GPPRRGASRG GGRGR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human WDR33 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: WDR33 (WD Repeat Domain 33 (WDR33))

Autre désignation: WDR33 (WDR33 Produits)

Synonymes: zgc:110570 Protein, WDR33 Protein, Wdr33 Protein, NET14 Protein, WDC146 Protein, 1110001N06Rik Protein, 2310011G05Rik Protein, 2810021O11Rik Protein, 8430413N20Rik Protein, WD repeat domain 33 Protein, pre-mRNA 3' end processing protein WDR33 Protein, wdr33 Protein, WDR33 Protein, Wdr33 Protein, LOC100548555 Protein

Sujet: Essential for both cleavage and polyadenylation of pre-mRNA 3' ends. {ECO:0000269|PubMed:19217410}.

Poids moléculaire: 146.7 kDa Including tag.

UniProt: Q9C0J8