XPO5 Protein (AA 2-1204) (His tag)

N° du produit ABIN3096411

Détail du produit

Antigène: XPO5 (Exportin 5 (XPO5))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1204

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette XPO5 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: AMDQVNALCE QLVKAVTVMM DPNSTQRYRL EALKFCEEFK EKCPICVPCG LRLAEKTQVA IVRHFGLQIL EHVVKFRWNG MSRLEKVYLK NSVMELIANG TLNILEEENH IKDALSRIVV EMIKREWPQH WPDMLIELDT LSKQGETQTE LVMFILLRLA EDVVTFQTLP PQRRRDIQQT LTQNMERIFS FLLNTLQENV NKYQQVKTDT SQESKAQANC RVGVAALNTL AGYIDWVSMS HITAENCKLL EILCLLLNEQ ELQLGAAECL LIAVSRKGKL EDRKPLMVLF GDVAMHYILS AAQTADGGGL VEKHYVFLKR LCQVLCALGN QLCALLGADS DVETPSNFGK YLESFLAFTT HPSQFLRSST QMTWGALFRH EILSRDPLLL AIIPKYLRAS MTNLVKMGFP SKTDSPSCEY SRFDFDSDED FNAFFNSSRA QQGEVMRLAC RLDPKTSFQM AGEWLKYQLS TFLDAGSVNS CSAVGTGEGS LCSVFSPSFV QWEAMTLFLE SVITQMFRTL NREEIPVNDG IELLQMVLNF DTKDPLILSC VLTNVSALFP FVTYRPEFLP QVFSKLFSSV TFETVEESKA PRTRAVRNVR RHACSSIIKM CRDYPQLVLP NFDMLYNHVK QLLSNELLLT QMEKCALMEA LVLISNQFKN YERQKVFLEE LMAPVASIWL SQDMHRVLSD VDAFIAYVGT DQKSCDPGLE DPCGLNRARM SFCVYSILGV VKRTCWPTDL EEAKAGGFVV GYTSSGNPIF RNPCTEQILK LLDNLLALIR THNTLYAPEM LAKMAEPFTK ALDMLDAEKS AILGLPQPLL ELNDSPVFKT VLERMQRFFS TLYENCFHIL GKAGPSMQQD FYTVEDLATQ LLSSAFVNLN NIPDYRLRPM LRVFVKPLVL FCPPEHYEAL VSPILGPLFT YLHMRLSQKW QVINQRSLLC GEDEAADENP ESQEMLEEQL VRMLTREVMD LITVCCVSKK GADHSSAPPA DGDDEEMMAT EVTPSAMAEL TDLGKCLMKH EDVCTALLIT AFNSLAWKDT LSCQRTTSQL CWPLLKQVLS GTLLADAVTW LFTSVLKGLQ MHGQHDGCMA SLVHLAFQIY EALRPRYLEI RAVMEQIPEI QKDSLDQFDC KLLNPSLQKV ADKRRKDQFK RLIAGCIGKP LGEQFRKEVH IKNLPSLFKK TKPMLETEVL DNDGGGLATI FEP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human XPO5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: XPO5 (Exportin 5 (XPO5))

Autre désignation: XPO5 (XPO5 Produits)

Synonymes: exp5 Protein, CG12234 Protein, Dmel\\CG12234 Protein, Exp-5 Protein, Exp5 Protein, RanBP21 Protein, dmExp5 Protein, 2410004H11Rik Protein, 2700038C24Rik Protein, AI648907 Protein, AW549301 Protein, RanBp21 Protein, mKIAA1291 Protein, exportin-5 Protein, ranbp21 Protein, DDBDRAFT_0185980 Protein, DDBDRAFT_0237583 Protein, DDB_0185980 Protein, DDB_0237583 Protein, XPO5 Protein, exportin 5 Protein, CG12234 gene product from transcript CG12234-RB Protein, armadillo-like helical domain-containing protein Protein, XPO5 Protein, Ranbp21 Protein, Xpo5 Protein, xpo5 Protein

Sujet: Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner., Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.

Poids moléculaire: 137.1 kDa Including tag.

UniProt: Q9HAV4

Pathways: Regulatory RNA Pathways, Protein targeting to Nucleus