ZNF658 Protein (AA 1-1059) (Strep Tag)

N° du produit ABIN3096526

Détail du produit

Antigène: ZNF658 (Zinc Finger Protein 658 (ZNF658))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1059

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ZNF658 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MNMSQASVSF QDVTVEFTRE EWQHLGPVER TLYRDVMLEN YSHLISVGYC ITKPKVISKL EKGEEPWSLE DEFLNQRYPG YFKVDHIKGI REKQEKPLWQ EIFISDADKT LSKEGQKVLE KPFNLEIAPE LSEKISCKCD SHRMNLPVAS QLIISERKYS RKKTEYMNVC EKLQLDIKHE KAHAEEKSYE HGENAKAFSY KKDQHWKFQT LEESFECDGS GQGLYDKTIC ITPQSFLTGE KSCKDDEFRK NFDKITLFNH MRTDTRGKCS DLNEYGTSCD KTTAVEYNKV HMAMTHYECN ERGINFSRKS PLTQSQRTIT GWSAFESNKC EENFSQSSAH IVHQKTQAGD KFGEHNECTD ALYQKLDFTA HQRIHTEDKF YLSDEHGKCR KSFYRKAHLI QHQRPHSGEK TYQYEECAKS FCSSSHPIQH PGTYVGFKLY ECNECGKAFC QNSNLSKHLR IHTKEKPCDN NGCGRSYKSP LIGHQKTDAE MELCGGSEYG KTSHLKGHQR ILMGEKPYEC IECGKTFSKT SHLRAHQRIH TGEKPYECVE CEKTFSHKTH LSVHQRVHTG EKPYECNDCG KSFTYNSALR AHQRIHTGEK PYECSDCEKT FAHNSALRAH HRIHTGEKPY ECNECGRSFA HISVLKAHQR IHTGEKPYEC NECGRSFTYN SALRAHQRIH TGRKPYECSD CEKTFAHNSA LKIHQRIHTG EKPYECNECE KTFAHNSALR AHQNIHTGEK LYECSECGKT FFQKTRLSTH RRIHTGEKPY ECSKCGKTFS QKSYLSGHER IHTGEKPYEC NVCGKTFVYK AALIVHQRIH TGEKPYECNQ CGKTFSQRTH LCAHQRIHTG EKPYECNECG KTFADNSALR AHHRIHTGEK PYECNDCGKT FSKTSHLRAH LRTRSGEKPY ECSECGKTFS EKSYVSAHQR VHTGEKPYEC NVCGKPFAHN STLRVHQRIH TGEKSYECND CGKTFSQKSH LSAHQRIHTG EKPYECNECG KAFAQNSTLR VHQRIHTGEK PYECDECGKT FVRKAALRVH HTRMHTREKT LACNGFGKS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ZNF658 (Zinc Finger Protein 658 (ZNF658))

Autre désignation: ZNF658 (ZNF658 Produits)

Synonymes: zinc finger protein 658 Protein, ZNF658 Protein

Sujet: Zinc finger protein 658,FUNCTION: Mediates transcriptional repression in response to zinc. Represses several genes, including SLC30A5, SLC30A10 and CBWD1, by binding to the zinc transcriptional regulatory element (ZTRE) (5'-C[AC]C[TAG]CC[TC]-N(0-50)-[GA]G[ATC]G[TG]G-3') found in the promoter region. May play a role in the control of ribosome biogenesis, regulating predominantly rRNA levels, as well as those of several ribosomal proteins, thus coordinating this highly zinc-demanding process with the available zinc supply. {ECO:0000269|PubMed:25582195}.

Poids moléculaire: 122.3 kDa

UniProt: Q5TYW1