Aquaporin 4 Protein (AQP4) (AA 1-323) (rho-1D4 tag,His tag)

N° du produit ABIN3089096

Détail du produit

Antigène: Aquaporin 4 (AQP4)

Type de proteíne: Recombinant

Attributs du protein: AA 1-323

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette Aquaporin 4 protéine est marqué à la rho-1D4 tag,His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: MTETSQVAPA GSSGHHHHHH SDRPTARRWG KCGPLCTREN IMVAFKGVWT QAFWKAVTAE FLAMLIFVLL SLGSTINWGG TEKPLPVDMV LISLCFGLSI ATMVQCFGHI SGGHINPAVT VAMVCTRKIS IAKSVFYIAA QCLGAIIGAG ILYLVTPPSV VGGLGVTMVH GNLTAGHGLL VELIITFQLV FTIFASCDSK RTDVTGSIAL AIGFSVAIGH LFAINYTGAS MNPARSFGPA VIMGNWENHW IYWVGPIIGA VLAGGLYEYV FCPDVEFKRR FKEAFSKAAQ QTKGSYMEVE DNRSQVETDD LILKPGVVHV IDVDRGEEKK GKDQSGEVLS SV
Sequence including N-terminal Rho1D4 tag and N-terminal His-tag.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human AQP4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. The protein is purified from the cleared cell lysate using His-tag capture materials.
2. Eluate fractions are analyzed by SDS-PAGE.
3. Protein containing fractions are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In solution (20 mM Hepes, pH 8.0, 150 mM NaCl, 0,8 % Octylglucopyranoside)

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Aquaporin 4 (AQP4)

Autre désignation: AQP4 (AQP4 Produits)

Synonymes: zgc:100922 Protein, si:ch211-192k9.1 Protein, AQP4 Protein, LOC100232616 Protein, HMIWC2 Protein, MIWC Protein, WCH4 Protein, AQP-4 Protein, AQP4.M23 Protein, MIWC2 Protein, Miwc Protein, aquaporin-4 Protein, aquaporin 4 Protein, aquaporin 4 L homeolog Protein, aqp4 Protein, AQP4 Protein, aqp4.L Protein, Aqp4 Protein

Sujet: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

Poids moléculaire: 36.8 kDa Including tag.

UniProt: P55087

Pathways: Sensory Perception of Sound