SLC1A7 Protein (AA 1-560) (Strep Tag)

N° du produit ABIN3109388

Détail du produit

Antigène: SLC1A7 (Solute Carrier Family 1 (Glutamate Transporter), Member 7 (SLC1A7))

Type de proteíne: Recombinant

Attributs du protein: AA 1-560

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SLC1A7 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MVPHAILARG RDVCRRNGLL ILSVLSVIVG CLLGFFLRTR RLSPQEISYF QFPGELLMRM LKMMILPLVV SSLMSGLASL DAKTSSRLGV LTVAYYLWTT FMAVIVGIFM VSIIHPGSAA QKETTEQSGK PIMSSADALL DLIRNMFPAN LVEATFKQYR TKTTPVVKSP KVAPEEAPPR RILIYGVQEE NGSHVQNFAL DLTPPPEVVY KSEPGTSDGM NVLGIVFFSA TMGIMLGRMG DSGAPLVSFC QCLNESVMKI VAVAVWYFPF GIVFLIAGKI LEMDDPRAVG KKLGFYSVTV VCGLVLHGLF ILPLLYFFIT KKNPIVFIRG ILQALLIALA TSSSSATLPI TFKCLLENNH IDRRIARFVL PVGATINMDG TALYEAVAAI FIAQVNNYEL DFGQIITISI TATAASIGAA GIPQAGLVTM VIVLTSVGLP TDDITLIIAV DWALDRFRTM INVLGDALAA GIMAHICRKD FARDTGTEKL LPCETKPVSL QEIVAAQQNG CVKSVAEASE LTLGPTCPHH VPVQVEQDEE LPAASLNHCT IQISELETNV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SLC1A7 (Solute Carrier Family 1 (Glutamate Transporter), Member 7 (SLC1A7))

Autre désignation: SLC1A7 (SLC1A7 Produits)

Synonymes: SLC1A7 Protein, si:ch211-284b7.1 Protein, slc1a7 Protein, A930031E15Rik Protein, Eaat5 Protein, AAAT Protein, EAAT5 Protein, solute carrier family 1 member 7 Protein, solute carrier family 1 (glutamate transporter), member 7a Protein, solute carrier family 1 (glutamate transporter), member 7 Protein, SLC1A7 Protein, slc1a7a Protein, Slc1a7 Protein

Sujet: Excitatory amino acid transporter 5 (Retinal glutamate transporter) (Solute carrier family 1 member 7),FUNCTION: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:9108121). Acts primarily as an inhibitory glutamate-gated chloride channel being a major inhibitory presynaptic receptor at mammalian rod bipolar cell axon terminals. Glutamate binding gates a large Cl(-) conductance that mediates inhibition, affecting visual processing in the retina (By similarity). {ECO:0000250|UniProtKB:Q8JZR4, ECO:0000269|PubMed:9108121}.

Poids moléculaire: 60.7 kDa

UniProt: O00341

Pathways: Dicarboxylic Acid Transport