Rho-related GTP-binding protein Protein (RhO (pan)) (AA 1-348)
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- Antigène Tous les produits Rho-related GTP-binding protein (RhO (pan))
- Rho-related GTP-binding protein (RhO (pan))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-348
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Origine
- Humain
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Source
- Cellules d'insectes
- Application
- SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
- Séquence
- MNGTEGPNFY VPFSNATGVV RSPFEYPQYY LAEPWQFSML AAYMFLLIVL GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVLGG FTSTLYTSLH GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE NHAIMGVAFT WVMALACAAP PLAGWSRYIP EGLQCSCGID YYTLKPEVNN ESFVIYMFVV HFTIPMIIIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV TRMVIIMVIA FLICWVPYAS VAFYIFTHQG SNFGPIFMTI PAFFAKSAAI YNPVIYIMMN KQFRNCMLTT ICCGKNPLGD DEASATVSKT ETSQVAPA
- Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human RHO Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
- The protein is purified from the cleared cell lysate using Rho1D4 capture materials.Eluate fractions are analyzed by SDS-PAGE.Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin-free.
- Classe de qualité
- Crystallography grade
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 40 mM Hepes, pH 6.8, 10 mM KCl, 130 mM NaCl,3 mM MgCl2, 2 mM CaCl2, 0,1 mM EDTA, 1 mM DTT, Leupeptin
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Rho-related GTP-binding protein (RhO (pan))
- Autre désignation
- rho (RhO (pan) Produits)
- Classe de substances
- Chemical
- Sujet
- Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
- Poids moléculaire
- 40.1 kDa Including tag.
- UniProt
- P08100
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