SLC16A10 Protein (AA 1-515) (Strep Tag)

N° du produit ABIN3116916

Détail du produit

Antigène: SLC16A10 (Solute Carrier Family 16, Member 10 (Aromatic Amino Acid Transporter) (SLC16A10))

Type de proteíne: Recombinant

Attributs du protein: AA 1-515

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SLC16A10 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MVLSQEEPDS ARGTSEAQPL GPAPTGAAPP PGPGPSDSPE AAVEKVEVEL AGPATAEPHE PPEPPEGGWG WLVMLAAMWC NGSVFGIQNA CGVLFVSMLE TFGSKDDDKM VFKTAWVGSL SMGMIFFCCP IVSVFTDLFG CRKTAVVGAA VGFVGLMSSS FVSSIEPLYL TYGIIFACGC SFAYQPSLVI LGHYFKKRLG LVNGIVTAGS SVFTILLPLL LRVLIDSVGL FYTLRVLCIF MFVLFLAGFT YRPLATSTKD KESGGSGSSL FSRKKFSPPK KIFNFAIFKV TAYAVWAVGI PLALFGYFVP YVHLMKHVNE RFQDEKNKEV VLMCIGVTSG VGRLLFGRIA DYVPGVKKVY LQVLSFFFIG LMSMMIPLCS IFGALIAVCL IMGLFDGCFI SIMAPIAFEL VGAQDVSQAI GFLLGFMSIP MTVGPPIAGL LRDKLGSYDV AFYLAGVPPL IGGAVLCFIP WIHSKKQREI SKTTGKEKME KMLENQNSLL SSSSGMFKKE SDSII
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SLC16A10 (Solute Carrier Family 16, Member 10 (Aromatic Amino Acid Transporter) (SLC16A10))

Autre désignation: SLC16A10 (SLC16A10 Produits)

Synonymes: MCT10 Protein, PRO0813 Protein, TAT1 Protein, Tat1 Protein, 2610103N14Rik Protein, 9830169E08 Protein, Mct10 Protein, solute carrier family 16 member 10 Protein, solute carrier family 16 (monocarboxylic acid transporters), member 10 Protein, SLC16A10 Protein, Slc16a10 Protein

Sujet: Monocarboxylate transporter 10 (MCT 10) (Aromatic amino acid transporter 1) (Solute carrier family 16 member 10) (T-type amino acid transporter 1),FUNCTION: Sodium- and proton-independent thyroid hormones and aromatic acids transporter (PubMed:11827462, PubMed:18337592, PubMed:28754537). Mediates both uptake and efflux of 3,5,3'-triiodothyronine (T3) and 3,5,3',5'-tetraiodothyronine (T4) with high affinity, suggesting a role in the homeostasis of thyroid hormone levels (PubMed:18337592). Responsible for low affinity bidirectional transport of the aromatic amino acids, such as phenylalanine, tyrosine, tryptophan and L-3,4-dihydroxyphenylalanine (L-dopa) (PubMed:11827462, PubMed:28754537). Plays an important role in homeostasis of aromatic amino acids (By similarity). {ECO:0000250|UniProtKB:Q3U9N9, ECO:0000269|PubMed:11827462, ECO:0000269|PubMed:18337592, ECO:0000269|PubMed:28754537}.

Poids moléculaire: 55.5 kDa

UniProt: Q8TF71