LPGAT1 Protein (AA 1-370) (Strep Tag)

N° du produit ABIN3117057

Détail du produit

Antigène: LPGAT1 (Lysophosphatidylglycerol Acyltransferase 1 (LPGAT1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-370

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette LPGAT1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAITLEEAPW LGWLLVKALM RFAFMVVNNL VAIPSYICYV IILQPLRVLD SKRFWYIEGI MYKWLLGMVA SWGWYAGYTV MEWGEDIKAV SKDEAVMLVN HQATGDVCTL MMCLQDKGLV VAQMMWLMDH IFKYTNFGIV SLVHGDFFIR QGRSYRDQQL LLLKKHLENN YRSRDRKWIV LFPEGGFLRK RRETSQAFAK KNNLPFLTNV TLPRSGATKI ILNALVAQQK NGSPAGGDAK ELDSKSKGLQ WIIDTTIAYP KAEPIDIQTW ILGYRKPTVT HVHYRIFPIK DVPLETDDLT TWLYQRFVEK EDLLSHFYET GAFPPSKGHK EAVSREMTLS NLWIFLIQSF AFLSGYMWYN IIQYFYHCLF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: LPGAT1 (Lysophosphatidylglycerol Acyltransferase 1 (LPGAT1))

Autre désignation: LPGAT1 (LPGAT1 Produits)

Synonymes: MGC68966 Protein, fc21c12 Protein, fj66b10 Protein, si:dkey-30h14.2 Protein, wu:fc21c12 Protein, wu:fj66b10 Protein, zgc:112278 Protein, FAM34A Protein, FAM34A1 Protein, NET8 Protein, AI649174 Protein, AW112037 Protein, BC013667 Protein, lysophosphatidylglycerol acyltransferase 1 S homeolog Protein, lysophosphatidylglycerol acyltransferase 1 Protein, lpgat1.S Protein, LPGAT1 Protein, lpgat1 Protein, Lpgat1 Protein

Sujet: Acyl-CoA:lysophosphatidylglycerol acyltransferase 1 (2-acylglycerophosphocholine O-acyltransferase) (EC 2.3.1.62) (Acyl-CoA:monoacylglycerol acyltransferase LPGAT1) (EC 2.3.1.22) (Lysophospholipid acyltransferase 7) (LPLAT7) (EC 2.3.1.-) (Stearoyl-CoA:1-lyso-2-acyl-PE acyltransferase),FUNCTION: Lysophospholipid acyltransferase involved in fatty acyl chain remodeling of glycerophospholipids in the endoplasmic reticulum membrane (By similarity). Selectively catalyzes the transfer and esterification of saturated long-chain fatty acids from acyl-CoA to the sn-1 position of 1-lyso-2-acyl phosphatidylethanolamines (1-lyso-PE, LPE), with a preference for stearoyl CoA over palmitoyl CoA as acyl donor (PubMed:36049524). Acts in concert with an unknown phospholipase A1 to convert palmitate phosphatidylethanolamine (PE) species into stearate ones. Provides substrates to the PE methylation pathway, controlling stearate/palmitate composition of PE and phosphatidylcholine (PC) species with an overall impact on de novo hepatic lipid synthesis, body fat content and life span (By similarity). Can acylate lysophosphatidylglycerols (LPG) using various saturated fatty acyl-CoAs as acyl donors (PubMed:15485873). Can also acylate monoacylglycerols with a preference for 2-monoacylglycerols over 1-monoacylglycerols (By similarity). Has no activity toward lysophosphatidic acids (LPA) (By similarity). {ECO:0000250|UniProtKB:Q91YX5, ECO:0000269|PubMed:15485873, ECO:0000269|PubMed:36049524}.

Poids moléculaire: 43.1 kDa

UniProt: Q92604