TMEM30A Protein (AA 2-361) (rho-1D4 tag)

N° du produit ABIN3118546

Détail du produit

Antigène: TMEM30A (Transmembrane Protein 30A (TMEM30A))

Type de proteíne: Recombinant

Attributs du protein: AA 2-361

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette TMEM30A protéine est marqué à la rho-1D4 tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: AMNYNAKDEV DGGPPCAPGG TAKTRRPDNT AFKQQRLPAW QPILTAGTVL PIFFIIGLIF IPIGIGIFVT SNNIREIEID YTGTEPSSPC NKCLSPDVTP CFCTINFTLE KSFEGNVFMY YGLSNFYQNH RRYVKSRDDS QLNGDSSALL NPSKECEPYR RNEDKPIAPC GAIANSMFND TLELFLIGND SYPIPIALKK KGIAWWTDKN VKFRNPPGGD NLEERFKGTT KPVNWLKPVY MLDSDPDNNG FINEDFIVWM RTAALPTFRK LYRLIERKSD LHPTLPAGRY SLNVTYNYPV HYFDGRKRMI LSTISWMGGK NPFLGIAYIA VGSISFLLGV VLLVINHKYR NSSNTADITI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TMEM30A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TMEM30A (Transmembrane Protein 30A (TMEM30A))

Autre désignation: TMEM30A (TMEM30A Produits)

Synonymes: tmem30a Protein, wu:fj66b08 Protein, zgc:91877 Protein, fi23a10 Protein, wu:fb15c10 Protein, wu:fi23a10 Protein, zgc:55379 Protein, zgc:77655 Protein, cg9947 Protein, MGC53259 Protein, MGC75889 Protein, CDC50A Protein, DKFZp459A091 Protein, DKFZp459D097 Protein, DKFZp459B0431 Protein, C6orf67 Protein, 2010200I23Rik Protein, AW540225 Protein, Cdc50a Protein, D9Wsu20e Protein, transmembrane protein 30Aa Protein, transmembrane protein 30A Protein, transmembrane protein 30Ab Protein, transmembrane protein 30A L homeolog Protein, tmem30aa Protein, TMEM30A Protein, tmem30ab Protein, tmem30a.L Protein, tmem30a Protein, LOAG_03933 Protein, Tmem30a Protein

Sujet: Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate. Required for the proper folding, assembly and ER to Golgi exit of the ATP8A2:TMEM30A flippase complex. ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth, and, reconstituted to liposomes, predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). The ATP8A1:TMEM30A flippase complex seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Required for the formation of the ATP8A2, ATP8B1 and ATP8B2 P-type ATPAse intermediate phosphoenzymes. Involved in uptake of platelet-activating factor (PAF), synthetic drug alkylphospholipid edelfosine, and, probably in association with ATP8B1, of perifosine. Also mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2, ATP8B4, ATP10A, ATP10B, ATP10D, ATP11A, ATP11B and ATP11C from the ER to other membrane localizations. {ECO:0000269|PubMed:20510206, ECO:0000269|PubMed:20947505, ECO:0000269|PubMed:20961850, ECO:0000269|PubMed:21289302}.

Poids moléculaire: 41.7 kDa Including tag.

UniProt: Q9NV96