B3GNT2 Protein (AA 29-325) (His tag)

N° du produit ABIN3120337

Détail du produit

Antigène: B3GNT2 (UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))

Type de proteíne: Recombinant

Attributs du protein: AA 29-325

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette B3GNT2 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: KNSSQDKNGK GGVIIPKEKF WKPPSTPRAY WNREQEKLNR WYNPILNRVA NQTGELATSP NTSHLSYCEP DSTVMTAVTD FNNLPDRFKD FLLYLRCRNY SLLIDQPKKC AKKPFLLLAI KSLIPHFARR QAIRESWGRE TNVGNQTVVR VFLLGKTPPE DNHPDLSDML KFESDKHQDI LMWNYRDTFF NLSLKEVLFL RWVSTSCPDA EFVFKGDDDV FVNTHHILNY LNSLSKSKAK DLFIGDVIHN AGPHRDKKLK YYIPEVFYTG VYPPYAGGGG FLYSGPLALR LYSATSR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse B3GNT2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: B3GNT2 (UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))

Autre désignation: B3GNT2 (B3GNT2 Produits)

Synonymes: B3GN-T2 Protein, B3GNT Protein, B3GNT-2 Protein, B3GNT1 Protein, BETA3GNT Protein, BGNT2 Protein, BGnT-2 Protein, AA408337 Protein, AA409404 Protein, AW260308 Protein, B3Galt6 Protein, B3gnt1 Protein, BGnT-1 Protein, BGnT1 Protein, BGnT2 Protein, Beta3gnt Protein, MGC81185 Protein, B3GNT2 Protein, MGC179257 Protein, ssp5 Protein, wu:fa55h04 Protein, wu:fc39c01 Protein, zgc:113947 Protein, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Protein, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 L homeolog Protein, beta-1,4-glucuronyltransferase 1 Protein, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2b Protein, B3GNT2 Protein, B3gnt2 Protein, b3gnt2.L Protein, B4GAT1 Protein, b3gnt2b Protein, b3gnt2 Protein

Sujet: Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains (PubMed:9892646). Probably constitutes the main polylactosamine synthase (PubMed:17890318). {ECO:0000269|PubMed:17890318, ECO:0000269|PubMed:9892646}.

Poids moléculaire: 35.2 kDa Including tag.

UniProt: Q9Z222

Pathways: Glycosaminoglycan Metabolic Process