EIF3S1 Protein (AA 1-261) (Strep Tag)

N° du produit ABIN3122019

Détail du produit

Antigène: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-261

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette EIF3S1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAAAAAAAAA AGDSDSWDAD TFSMEDPVRK VAGGGTAGGD RWEGEDEDED VKDNWDDDDD ENKEEAEVKP EVKISEKKKI AEKIKEKERQ QKKRQEEIKK RLEEPEESKV LTPEEQLADK LRLKKLQEES DLELAKETFG VNNTVYGIDA MNPSSRDDFT EFGKLLKDKI TQYEKSLYYA SFLEALVRDV CISLEIDDLK KITNSLTVLC SEKQKQEKQS KAKKKKKGVV PGGGLKATMK DDLADYGGYE GGYVQDYEDF M
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Autre désignation: Eif3j1 (EIF3S1 Produits)

Synonymes: GB12381 Protein, An18g06260 Protein, AO090003000054 Protein, EIF3S1 Protein, eIF3j Protein, eIF3-alpha Protein, eIF3-p35 Protein, Eif3s1 Protein, eif3s1 Protein, 1810016I04Rik Protein, 2700079K05Rik Protein, AA409117 Protein, AA409446 Protein, C78575 Protein, Eif3j Protein, Eif3j-1 Protein, Eif3j-2 Protein, Eif3j2 Protein, eIF-3-alpha-A Protein, eIF-3-alpha-B Protein, eIF3j-A Protein, eIF3j-B Protein, eukaryotic translation initiation factor 3 subunit J Protein, eukaryotic translation initiation factor 3, subunit J Protein, Eukaryotic translation initiation factor 3 subunit J Protein, eukaryotic translation initiation factor 3 subunit 1 Protein, eukaryotic translation initiation factor 3 subunit J S homeolog Protein, eukaryotic translation initiation factor 3, subunit J1 Protein, LOC409796 Protein, ANI_1_830164 Protein, AOR_1_94154 Protein, Eif3j Protein, EIF3J Protein, eif3j Protein, LOC733089 Protein, eif3j.S Protein, Eif3j1 Protein

Sujet: Eukaryotic translation initiation factor 3 subunit J-A (eIF3j-A) (Eukaryotic translation initiation factor 3 subunit 1-A) (eIF-3-alpha-A) (eIF3 p35),FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA. {ECO:0000255|HAMAP-Rule:MF_03009}.

Poids moléculaire: 29.3 kDa

UniProt: Q3UGC7

Pathways: Ribonucleoprotein Complex Subunit Organization