CYBB Protein (AA 283-570) (His tag)

N° du produit ABIN3122850

Détail du produit

Antigène: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Type de proteíne: Recombinant

Attributs du protein: AA 283-570

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette CYBB protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: ERLVRFWRSQ QKVVITKVVT HPFKTIELQM KKKGFKMEVG QYIFVKCPKV SKLEWHPFTL TSAPEEDFFS IHIRIVGDWT EGLFNACGCD KQEFQDAWKL PKIAVDGPFG TASEDVFSYE VVMLVGAGIG VTPFASILKS VWYKYCDNAT SLKLKKIYFY WLCRDTHAFE WFADLLQLLE TQMQERNNAN FLSYNIYLTG WDESQANHFA VHHDEEKDVI TGLKQKTLYG RPNWDNEFKT IASEHPNTTI GVFLCGPEAL AETLSKQSIS NSESGPRGVH FIFNKENF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cybb Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Autre désignation: Cybb (CYBB Produits)

Synonymes: zgc:64144 Protein, cgd Protein, gp91-1 Protein, gp91-phox Protein, gp91phox Protein, nox2 Protein, NOX2 Protein, cybb Protein, NOX1 Protein, AMCBX2 Protein, CGD Protein, GP91-1 Protein, GP91-PHOX Protein, GP91PHOX Protein, p91-PHOX Protein, Nox2 Protein, C88302 Protein, CGD91-phox Protein, Cgd Protein, Cyd Protein, Gp91-phox Protein, CYBB Protein, cytochrome b-245, beta polypeptide (chronic granulomatous disease) Protein, cytochrome b-245, beta polypeptide Protein, cytochrome b-245 heavy chain Protein, cytochrome b-245, beta polypeptide L homeolog Protein, cytochrome b-245 beta polypeptide Protein, cytochrome b-245 beta chain Protein, NADPH oxidase heavy chain subunit Protein, cybb Protein, CYBB Protein, cybb.L Protein, Cybb Protein, GP91-PHOX Protein

Sujet: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. {ECO:0000269|PubMed:24739962}.

Poids moléculaire: 34.2 kDa Including tag.

UniProt: Q61093