TAF5L Protein (AA 1-589) (Strep Tag)

N° du produit ABIN3127392

Détail du produit

Antigène: TAF5L (TAF5-Like RNA Polymerase II, P300/CBP-Associated Factor (PCAF)-Associated Factor, 65kDa (TAF5L))

Type de proteíne: Recombinant

Attributs du protein: AA 1-589

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TAF5L protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MKRVRTEQVQ VAVSCYLKRR QYVDSEGPLK QGLRLSQTPE EMAANLTVQS ESGCANAVSA APCQAEPQQY EVQFGRLRSF LTDSDSQYSR EVMPLLYPLF VYLHLNLVQS GPKSTVESFY SRFHGMFLQN ASQKDVIEQL QTTQTIQDIL SNFQLRAFLD NKYVVRLQED SYNYLIRYLQ SDNNTALCKV LAVHIHLDVQ PAKRTDYQLY ASGGSSRTEN SSLEPPEVPS PILQNEAALE VLQESIKRVK DGPPSLTTIC FYAFYNTEQL LNTAEISSDS KLLAAGFDNS CIKLWSLRSK KLKSEPHHVD TSRIRLACDT LEEEENEEDN TGTEMKILRG HCGPVYSTRF LADSSGLLSC SEDMSIRYWD LGSFTNTVLY QGHAYPVWDV DISPFSLYFA SGSHDRTARL WSFDRTYPLR IYAGHLADVD CVKFHPNSNY LATGSTDKTV RLWSAQQGNS VRLFTGHRGP VLSLSFSPNG KYLASAGEDQ RLKLWDLASG TLFKELRGHT DSITSLAFSP DSGLIASASM DNSVRVWDIR STCCNTPADG SSGELVGVYT GQMSNVLSVQ FMACNLLLVT GITQENQEH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TAF5L (TAF5-Like RNA Polymerase II, P300/CBP-Associated Factor (PCAF)-Associated Factor, 65kDa (TAF5L))

Autre désignation: Taf5l (TAF5L Produits)

Synonymes: PAF65B Protein, taf5l Protein, MGC89568 Protein, fi28g02 Protein, wu:fi28g02 Protein, zgc:63765 Protein, 1110005N04Rik Protein, AI849020 Protein, TATA-box binding protein associated factor 5 like Protein, TATA-box binding protein associated factor 5 like L homeolog Protein, PCAF associated factor 65 beta Protein, TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor Protein, TAF5L Protein, taf5l.L Protein, taf5l Protein, PY07202 Protein, Taf5l Protein

Sujet: TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L (TAF5L) (PCAF-associated factor 65 beta) (PAF65-beta),FUNCTION: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex (By similarity). With TAF6L, acts as an epigenetic regulator essential for somatic reprogramming. Regulates target genes through H3K9ac deposition and MYC recruitment which trigger MYC regulatory network to orchestrate gene expression programs to control embryonic stem cell state (PubMed:31005419). {ECO:0000250|UniProtKB:O75529, ECO:0000269|PubMed:31005419}.

Poids moléculaire: 65.9 kDa

UniProt: Q91WQ5